小地老虎中两个几丁质合酶基因的鉴定与功能分析

Identification and Functional Analysis of Two Chitin Synthase Genes in the Common Cutworm, .

作者信息

Yu Hai-Zhong, Li Ning-Yan, Xie Yan-Xin, Zhang Qin, Wang Ying, Lu Zhan-Jun

机构信息

School of Life Sciences, Gannan Normal University, Ganzhou 341000, China.

National Navel Orange Engineering and Technology Research Center, Gannan Normal University, Ganzhou 341000, China.

出版信息

Insects. 2020 Apr 17;11(4):253. doi: 10.3390/insects11040253.

Chitin is one the main components of the insect cuticle, and chitin synthase (CHS) is an important enzyme required for chitin formation. CHS has been characterized in various insect species, but the structure and biochemical properties in have not been determined. In this study, we identified two genes, and , which encode proteins with 1565 and 1520 amino acid residues, respectively. Transcriptional analysis suggested that has a high expression level in the integument whereas showed the highest expression level in the midgut. During growth and development, and were both predominantly expressed in the fourth-instar larval stage. In addition, the expression of and could be induced by 20-hydroxyecdysone (20E). Silencing of by RNA interference significantly inhibited the pupation and molting of larvae (RNAi), while knockdown of had no significant effects on the phenotype. These results may provide a new molecular target for control of .

几丁质是昆虫表皮的主要成分之一，几丁质合成酶（CHS）是几丁质形成所需的一种重要酶。CHS已在多种昆虫物种中得到表征，但在[此处原文缺失相关物种信息]中的结构和生化特性尚未确定。在本研究中，我们鉴定出两个[此处原文缺失基因名称]基因，分别编码具有1565个和1520个氨基酸残基的蛋白质。转录分析表明，[此处原文缺失第一个基因名称]在体壁中具有高表达水平，而[此处原文缺失第二个基因名称]在中肠中表达水平最高。在[此处原文缺失相关昆虫名称]生长发育过程中，[此处原文缺失第一个基因名称]和[此处原文缺失第二个基因名称]均主要在四龄幼虫阶段表达。此外，[此处原文缺失第一个基因名称]和[此处原文缺失第二个基因名称]的表达可被20-羟基蜕皮激素（20E）诱导。通过RNA干扰沉默[此处原文缺失第一个基因名称]显著抑制了[此处原文缺失相关昆虫名称]幼虫的化蛹和蜕皮（RNAi），而敲低[此处原文缺失第二个基因名称]对[此处原文缺失相关昆虫名称]的表型没有显著影响。这些结果可能为[此处原文缺失相关昆虫名称]的防治提供新的分子靶点。