Department of Forensic Medicine, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, Xinjiang 830017, China.
Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, Hunan 410013, China.
J Insect Sci. 2023 Nov 1;23(6). doi: 10.1093/jisesa/iead107.
Chitinases play a crucial role in insect metamorphosis by facilitating chitin degradation. Sarcophaga peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) is a typical holometabolous insect and an important hygiene pest that causes myiasis in humans and other mammals and acts as a vector for various parasitic agents, including bacteria, viruses, and parasites. Enhancing the understanding of the metamorphosis in this species has significance for vector control. In this study, we identified a total of 12 chitinase genes in S. peregrina using bioinformatic analysis methods. Based on transcriptome data, SpIDGF2 and SpCht10 were selected for further functional investigation. The down-regulation of these genes by RNA interference led to developmental delays, disruptions in molting, and differences in cuticle composition during the pupal stage. These findings underscore the pivotal role of chitinase genes in the metamorphic process and offer valuable insights for effective control strategies.
几丁质酶在昆虫变态过程中发挥着关键作用，有助于几丁质的降解。麻蝇（Sarcophaga peregrina）（双翅目：麻蝇科）是一种典型的完全变态昆虫，也是一种重要的卫生害虫，它会引起人类和其他哺乳动物的蝇蛆病，并作为各种寄生虫，包括细菌、病毒和寄生虫的载体。深入了解该物种的变态过程对于控制媒介具有重要意义。在本研究中，我们使用生物信息学分析方法在麻蝇中总共鉴定出 12 种几丁质酶基因。基于转录组数据，选择 SpIDGF2 和 SpCht10 进行进一步的功能研究。这些基因的 RNA 干扰下调导致发育延迟、蜕皮破坏以及蛹期表皮成分的差异。这些发现强调了几丁质酶基因在变态过程中的关键作用，并为有效的控制策略提供了有价值的见解。
