Watts M E
Int J Radiat Biol Relat Stud Phys Chem Med. 1977 Mar;31(3):237-50. doi: 10.1080/09553007714550281.
The radiosensitizer nifurpipone dihydrochloride (5-nitro-2-furaldehyde N-methyl piperazino acetyl hydrazone dihydrochloride) sensitizes hypoxic V79 mammalian cells by at least two mechanisms. Sensitization is by a reduction of ñ in addition to an increase in slope. Both these affects are absent under oxygenated conditions. When hypoxic V79 cells are irradiated in the presence of nifurpipone dihydrochloride combined with Ro-07-0582, sensitization greater than that due to air alone is observed; this effect is due to a reduction in ñ and an increased slope. Again this effect is absent under oxygenated conditions. Rapid-mix studies using Serratia marcescens show that full senitization occurs with a pre-irradiation contact time of 4 msec; this contrasts with data for V79 cells where a pre-irradiation contact time of 40 msec is insufficient for any sensitization to occur. This sensitizer also exerts a differential toxic effect, being more toxic to hypoxic cells than to oxygenated ones. It is concluded from these results that nifurpipone dihydrochloride sensitizes by at least two mechanisms, one of which resembles that of the electron-affinic type.
放射增敏剂盐酸硝呋哌酮（5-硝基-2-糠醛N-甲基哌嗪乙酰腙二盐酸盐）通过至少两种机制使缺氧的V79哺乳动物细胞增敏。除了斜率增加外，增敏作用还通过降低ñ来实现。在有氧条件下，这两种影响均不存在。当在盐酸硝呋哌酮与Ro-07-0582联合存在的情况下照射缺氧的V79细胞时，观察到的增敏作用大于仅在空气中照射时的增敏作用；这种效应是由于ñ降低和斜率增加所致。同样，在有氧条件下这种效应不存在。使用粘质沙雷氏菌进行的快速混合研究表明，预照射接触时间为4毫秒时会发生完全增敏；这与V79细胞的数据形成对比，在V79细胞中，预照射接触时间为40毫秒不足以产生任何增敏作用。这种增敏剂还具有不同的毒性作用，对缺氧细胞的毒性比对有氧细胞的毒性更大。从这些结果可以得出结论，盐酸硝呋哌酮通过至少两种机制增敏，其中一种机制类似于亲电子型机制。
