CCR4 Skin-Tropic Phenotype as a Feature of Central Memory CD8 T Cells in Healthy Subjects and Psoriasis Patients.

The chemokine receptor CCR4 has emerged as a skin-homing molecule important for the migration of T cells from the blood to the dermis. From our previous data on psoriasis patients, CCR4 memory T cells emerged as a putative recirculating population between skin and blood. Here we focused our attention on the expression of CCR4 and skin-tropic molecules in the different stages of memory T cell differentiation. We analyzed the chemokine receptor profile in CD8 and CD4 CD45RACCR7 (T) and CD45RACCR7 (T) cells. Subpopulations were further divided on the basis of CD62L expression, and the distribution among the subsets of the skin-homing molecule CLA (Cutaneous Lymphocyte Antigen) was evaluated. The characterization was performed on peripheral blood mononuclear cells isolated from 21 healthy subjects and 24 psoriasis patients. The results indicate that (i) the skin-homing CCR4 marker is mainly expressed in T cells, (ii) CCR4 T cells also express high level of CLA and that (iii) the more differentiated phenotype T expresses CXCR3 and CCR5 but lower level of CCR4 and CLA. This indicates that progressive stages of memory T cell differentiation have profoundly different chemokine receptor patterns, with CD8 T displaying a marked skin-tropic phenotype CLACCR4. Differential skin-tropic phenotype between T and T cells was observed in both healthy subjects and psoriasis patients. However, patients showed an expanded circulating population of CD8 T cells with phenotype CCR4CXCR3 that could play a role in the pathophysiology of psoriasis and possibly in disease recurrence.