抗氧化治疗后复发性流产夫妇精子参数与染色质的比较。
Comparison between sperm parameters and chromatin in recurrent pregnancy loss couples after antioxidant therapy.
作者信息
Nazari Alireza, Sabeti Parvin, Pourmasumi Soheila
机构信息
Non-Communicable Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
Clinical Research Development Unit (CRDU), Moradi Hospital, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
出版信息
J Family Med Prim Care. 2020 Feb 28;9(2):597-601. doi: 10.4103/jfmpc.jfmpc_1105_19. eCollection 2020 Feb.
BACKGROUND AND AIMS
Recurrent Pregnancy loss (RPL) is a heterogeneous disease. The role of maternal factor is clear but the relationship between the paternal factors remains uncertain. It has been shown that increase the level of Reactive Oxygen Species (ROS) and decrease the antioxidant levels in men can lead to RPL. New researches show treatment with antioxidant can improved sperm parameter. The aim of the present study was to assess the effect of vitamin E plus Zinc therapy on sperm parameters and chromatin quality in couples with RPL.
METHODS
In this clinical trial study, 60 RPL patients were selected from RPL clinic. Standard sperm parameters were analyzed and then male partners were intake vitamin E and Zinc in antioxidant therapy plan for 90 days. After that, sperm parameters were analyzed again. Sperm chromatin assay was reviewed before and after vitamin E and Zinc therapy by cytochemical assay including aniline blue (AB), chromomycin A3 (CMA3), toluidine blue (TB). To assess DNA fragmentation index, TUNEL test was used. Data were analyzed and compared before and after treatment.
RESULTS
data analysis showed all sperm parameters significantly improved after treatment ( < 0.001). The number of AB and TB sperms were decreased significantly after vitamin E and Zinc therapy ( = 0.0001). Decrease in DNA fragmentation in post treatment group in comparison to pre-treatment was statistically significant.
CONCLUSION
Supplemental of vitamin E plus Zinc may improve sperm parameters chromatin quality and decrease sperm DNA fragmentation in RPL patients.
背景与目的
复发性流产（RPL）是一种异质性疾病。母体因素的作用已明确，但父体因素之间的关系仍不确定。研究表明，男性体内活性氧（ROS）水平升高和抗氧化剂水平降低会导致复发性流产。新研究表明，抗氧化剂治疗可改善精子参数。本研究的目的是评估维生素E加锌疗法对复发性流产夫妇精子参数和染色质质量的影响。
方法
在这项临床试验研究中，从复发性流产诊所选取60例复发性流产患者。分析标准精子参数，然后男性伴侣按照抗氧化治疗方案摄入维生素E和锌，持续90天。之后，再次分析精子参数。通过细胞化学分析，包括苯胺蓝（AB）、放线菌素A3（CMA3）、甲苯胺蓝（TB），在维生素E和锌治疗前后对精子染色质进行检测。为评估DNA碎片化指数，使用了TUNEL检测。对治疗前后的数据进行分析和比较。
结果
数据分析显示，治疗后所有精子参数均显著改善（<0.001）。维生素E和锌治疗后，AB和TB精子数量显著减少（=0.0001）。与治疗前相比，治疗后组DNA碎片化减少具有统计学意义。
结论
补充维生素E加锌可能改善复发性流产患者的精子参数、染色质质量并减少精子DNA碎片化。
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