Center of Translational Medicine, Zibo Central Hospital, Shandong University, Zibo 255036, China.
McKusick-Zhang Center for Genetic Medicine, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
Acta Biochim Biophys Sin (Shanghai). 2020 May 26;52(5):563-569. doi: 10.1093/abbs/gmaa027.
Osteoblast differentiation is a key process in bone homeostasis. Mutations in plastin 3 have been reported to be responsible for X-linked osteoporosis. Plastin 3 and plastin 2 act synergistically to regulate osteoblast differentiation. However, the bone-related function of plastin 1, another family member of plastins, has not been assessed. In this study, we addressed the functional importance of plastin 1 in osteoblasts. We characterized the expression patterns of plastin 1 during osteoblast differentiation and revealed its important role in this process. In both HEK 293T and hFOB1.19 cells, plastin 1 was demonstrated to regulate intracellular Ca2+. Accordingly, we revealed that higher Ca2+ concentration promotes osteoblast differentiation. Finally, we found that plastin 1 may play a compensatory role in osteoporosis patients with plastin 3 deficiency. Together, our results indicate that plastin 1 promotes osteoblast differentiation by regulating intracellular Ca2+. Our work sheds new light on the role played by plastins in bone homeostasis.
成骨细胞分化是骨内稳态的关键过程。已有报道称，塑性 3 的突变可导致 X 连锁骨质疏松症。塑性 3 和塑性 2 协同作用调节成骨细胞分化。然而，塑性家族的另一个成员塑性 1 的骨相关功能尚未得到评估。在这项研究中，我们研究了塑性 1 在成骨细胞中的功能重要性。我们描述了塑性 1 在成骨细胞分化过程中的表达模式，并揭示了它在这一过程中的重要作用。在 HEK 293T 和 hFOB1.19 细胞中，塑性 1 被证明调节细胞内 Ca2+。相应地，我们揭示了较高的 Ca2+浓度促进成骨细胞分化。最后，我们发现塑性 1 可能在塑性 3 缺乏的骨质疏松症患者中发挥代偿作用。总之，我们的结果表明，塑性 1 通过调节细胞内 Ca2+促进成骨细胞分化。我们的工作为塑性在骨内稳态中的作用提供了新的认识。
