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使用针对高度纯化的肌动蛋白和肌球蛋白的抗体，对豚鼠睾丸曲细精管细胞中的收缩蛋白进行直接可视化观察。

Direct visualization of contractile proteins in pertubular cells of the guinea-pig testis using antibodies against highly purified actin and myosin.

作者信息

Gröshel-Stewart U, Unsicker K

出版信息

Histochemistry. 1977 Apr 4;51(4):315-9. doi: 10.1007/BF00494367.

DOI:10.1007/BF00494367
PMID:323203
Abstract

Antibodies against actin and myosin from smooth muscle, which may react with contractile elements from both muscular and muscle-like cells, were applied to fresh frozen sections of adult guinea-pig testis. Sections stained with an antibody against pectoralis (striated) muscle myosin or with non-immune globulin were used for controls. Peritubular cells of the lamina propria surrounding seminiferous tubulus contained large amounts of actin and myosin as judged by the intensity of immunofluorescence. Sertoli cells did not stain with the antibodies. Our results support the concept of peritubular cells being the critical force for the contractility of seminiferous tubules.

摘要

针对平滑肌肌动蛋白和肌球蛋白的抗体，可能与肌肉细胞和类肌肉细胞的收缩成分发生反应，将其应用于成年豚鼠睾丸的新鲜冷冻切片。用抗胸肌（横纹肌）肌球蛋白抗体或非免疫球蛋白染色的切片用作对照。根据免疫荧光强度判断，生精小管周围固有层的管周细胞含有大量肌动蛋白和肌球蛋白。支持细胞未被抗体染色。我们的结果支持管周细胞是生精小管收缩关键力量的概念。

相似文献

1
Direct visualization of contractile proteins in pertubular cells of the guinea-pig testis using antibodies against highly purified actin and myosin.使用针对高度纯化的肌动蛋白和肌球蛋白的抗体，对豚鼠睾丸曲细精管细胞中的收缩蛋白进行直接可视化观察。
Histochemistry. 1977 Apr 4;51(4):315-9. doi: 10.1007/BF00494367.
2
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Cell Tissue Res. 1978 Feb 14;187(1):97-103. doi: 10.1007/BF00220621.
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Characterization of rat testicular peritubular myoid cells in culture: alpha-smooth muscle isoactin is a specific differentiation marker.培养的大鼠睾丸生精小管周肌样细胞的特性：α-平滑肌同功肌动蛋白是一种特异性分化标志物。
Biol Reprod. 1990 Feb;42(2):351-65. doi: 10.1095/biolreprod42.2.351.
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Distribution of contractile proteins and adrenergic nerves in the adrenal gland of guinea-pig, rat and ox as revealed by immunofluorescence and the glyoxylic acid technique.通过免疫荧光和乙醛酸技术揭示豚鼠、大鼠和牛肾上腺中收缩蛋白和肾上腺素能神经的分布。
Experientia. 1978 Jan 15;34(1):102-5. doi: 10.1007/BF01921929.
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Correlation between the distribution of smooth muscle or non muscle myosins and alpha-smooth muscle actin in normal and pathological soft tissues.正常及病理软组织中平滑肌或非肌肉肌球蛋白分布与α-平滑肌肌动蛋白之间的相关性
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Specific binding of anti-myosin and -actin gamma-globulins to the surface of trypsin-dissociated embryonic chick cells.抗肌球蛋白和抗肌动蛋白γ球蛋白与胰蛋白酶解离的鸡胚细胞表面的特异性结合。
Cell Tissue Res. 1976 Aug 26;171(3):351-8. doi: 10.1007/BF00224659.
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Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. I. Antibodies to smooth and striated chicken muscle myosins.收缩蛋白特异性抗体的产生及其在免疫荧光显微镜中的应用。I. 鸡平滑肌和横纹肌肌球蛋白抗体。
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引用本文的文献

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Xenografting of isolated equine (Equus caballus) testis cells results in de novo morphogenesis of seminiferous tubules but not spermatogenesis.移植分离出的马（马属马种）睾丸细胞可导致生精小管的新生形态发生，但不能产生精子。
Andrology. 2017 Mar;5(2):336-346. doi: 10.1111/andr.12308. Epub 2017 Feb 3.
2
Comparison of purified anti-actin and fluorescent-heavy meromyosin staining patterns in dividing cells.分裂细胞中纯化的抗肌动蛋白和荧光重酶解肌球蛋白染色模式的比较。
J Cell Biol. 1979 Mar;80(3):509-20. doi: 10.1083/jcb.80.3.509.

本文引用的文献

1
Study of normal and irradiated boundary tissue of the seminiferous tubules of the rat.大鼠生精小管正常及受辐照边界组织的研究。
Exp Cell Res. 1960 Oct;21:49-70. doi: 10.1016/0014-4827(60)90346-3.
2
Contractile elements in the limiting membrane of the seminiferous tubules of the rat.大鼠生精小管界膜中的收缩成分。
Exp Cell Res. 1958 Oct;15(2):438-40. doi: 10.1016/0014-4827(58)90052-1.
3
Contractile cells in human seminiferous tubules.人类生精小管中的收缩细胞。
Science. 1966 Sep 9;153(3741):1271-3. doi: 10.1126/science.153.3741.1271.
4
The fine structure and development of the peritubular contractile cell component in the seminiferous tubules of the mouse.小鼠生精小管中肾小管周收缩细胞成分的精细结构与发育
Am J Anat. 1967 Nov;121(3):523-57. doi: 10.1002/aja.1001210307.
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Effect of androgens and antiandrogens on the development of the myoid cells of the rat seminiferous tubules.雄激素和抗雄激素对大鼠生精小管肌样细胞发育的影响。
Z Zellforsch Mikrosk Anat. 1972;131(3):299-308. doi: 10.1007/BF00582853.
6
Lymph vascular system of the interstitial tissue of the testis as revealed by electron microscopy.睾丸间质组织淋巴管系统的电镜观察
J Reprod Fertil. 1969 Jun;19(1):109-19. doi: 10.1530/jrf.0.0190109.
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Contractile filamentous structures in Sertoli cells of the Greek tortoise (Testudo graeca)?
Experientia. 1974 Mar 15;30(3):272-3. doi: 10.1007/BF01934823.
8
Electron microscopy of filaments in the basal part of rat kidney tubule cells, and their in situ interaction with heavy meromyosin.大鼠肾小管细胞基部细丝的电子显微镜观察及其与重酶解肌球蛋白的原位相互作用。
Z Zellforsch Mikrosk Anat. 1972;132(4):497-521. doi: 10.1007/BF00306638.
9
[Contractile fibroblasts (myofibroblasts) in the lamina propria of human seminiferous tubules].
Z Zellforsch Mikrosk Anat. 1972;133(4):519-27.
10
[Light and electron microscopic studies on the porcine testicular peritubular cells during postnatal development (author's transl)].仔猪出生后发育过程中睾丸生精小管周围细胞的光镜与电镜研究（作者译）
Z Anat Entwicklungsgesch. 1973 Dec 31;143(1):49-64.