Gröschel-Stewart U, Schreiber J, Mahlmeister C
Histochemistry. 1976 Feb 26;46(3):229-36. doi: 10.1007/BF02462786.
Antibodies prepared against actomyosins can be shown to behave similarly, if not identically to more recently prepared antibodies against highly purified myosins. Details of the purification of the antigens, and of the production of antibodies to chick myosins from smooth gizzard muscle and from striated pectoral muscle are given. The antibody specificity appears to be directed against the heavy chains of the myosin molecules, since these antibodies specifically inhibit the myosin ATPase reaction, and since in situ staining of myosin polypeptide chains on an SDS gel using the antibodies in indirect fluorescence shows staining only in the heavy band region. Use of the antibodies in immunofluorescence microscopy suggest that the antibodies are tissue, but not species, specific. Example of their use in staining tissue sections are shown.
针对肌动球蛋白制备的抗体，即便与最近针对高度纯化的肌球蛋白制备的抗体不完全相同，其表现也相似。文中给出了抗原纯化的细节，以及从平滑肌胃和横纹胸肌中制备鸡肌球蛋白抗体的细节。抗体特异性似乎针对肌球蛋白分子的重链，因为这些抗体能特异性抑制肌球蛋白ATP酶反应，并且在SDS凝胶上使用抗体进行间接荧光原位染色时，仅在重链区域显示染色。免疫荧光显微镜中使用这些抗体表明，这些抗体具有组织特异性，但不具有物种特异性。文中展示了它们用于染色组织切片的示例。
