Ubani-Ukoma Uloma, Chauhan Anuj, Schultz Gregory, Gibson Daniel J
Department of Pharmaceutics & Pharmaceutical Technology, Faculty of Pharmacy, University of Lagos, Nigeria.
Department of Chemical and Biological Engineering, Colorado School of Mines, United States.
MethodsX. 2020 Apr 7;7:100876. doi: 10.1016/j.mex.2020.100876. eCollection 2020.
In vitro screening and testing of drugs and devices is necessary, but in vitro conditions differ greatly from those found in vivo. These differences can lead to false promises of efficacy, or can hide problems of tissue compatibility. Models with tissues can be highly valuable bridges which provide relevant matrices for testing [1], [2], [3], [4], [5], [6], [7], [8], [9]. tissue models which are closer both biochemically and biophysically can provide useful feedback in a more time- and cost-efficient manner. Herein we describe an corneal model for use in drug delivery testing and corneal infection modeling [10]. The protocol covers the tissue harvesting, sterilization, inoculation, and bacterial load quantification. We envision that the model can be used to study bacterial physiology on metabolizable matrices and to study the direct effects of microbial colonization on the cornea's integrity and clarity.•Devitalized cornea.•Non-submersed conditions.•Contact lens compatible.
药物和器械的体外筛选与测试是必要的，但体外条件与体内条件差异很大。这些差异可能导致对疗效的虚假承诺，或者掩盖组织相容性问题。带有组织的模型可以成为非常有价值的桥梁，为测试提供相关基质[1,2,3,4,5,6,7,8,9]。在生化和生物物理方面更接近的组织模型可以以更省时、更经济高效的方式提供有用的反馈。在此，我们描述一种用于药物递送测试和角膜感染建模的角膜模型[10]。该方案涵盖组织采集、灭菌、接种和细菌载量定量。我们设想该模型可用于研究可代谢基质上的细菌生理学，以及研究微生物定植对角膜完整性和透明度的直接影响。
•失活角膜。
•非浸没条件。
•与隐形眼镜兼容。
