Tian Qing, Guo Shi-Meng, Xie Shi-Ming, Yin Ying, Zhou Li-Quan
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei, China.
School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology , Wuhan, Hubei, China.
Cell Cycle. 2020 Jun;19(12):1492-1501. doi: 10.1080/15384101.2020.1754585. Epub 2020 Apr 23.
Ring1 and Yin Yang 1-Binding Protein (RYBP) is a member of non-canonical polycomb repressive complex 1 to mediate monoubiquitination of histone H2A at lysine 119. It plays an important role in development, but its role in reproduction remains illusive. In this study, we used Rybp conditional knockout mouse model to genetically ablate Rybp in male germ cells. We found that Rybp deficiency during spermatogenesis led to smaller testes, loss of germline cells, disturbed meiosis, increased apoptosis of spermatocytes, decreased sperm motility, and reduced global H3K9me3, without impacting retrotransposon expression. Meanwhile, we depleted Rybp during oogenesis, but oocyte maturation and preimplantation development were normal. Our findings demonstrate that RYBP plays important roles in spermatogenesis through regulating meiosis and sperm motility.
Ring1与阴阳1结合蛋白（RYBP）是非经典多梳抑制复合物1的成员，可介导组蛋白H2A赖氨酸119位点的单泛素化。它在发育过程中发挥重要作用，但其在生殖中的作用仍不清楚。在本研究中，我们使用Rybp条件性敲除小鼠模型在雄性生殖细胞中基因敲除Rybp。我们发现，精子发生过程中Rybp缺乏导致睾丸变小、生殖细胞丢失、减数分裂紊乱、精母细胞凋亡增加、精子活力下降以及整体H3K9me3减少，而不影响逆转座子表达。同时，我们在卵子发生过程中耗尽Rybp，但卵母细胞成熟和植入前发育正常。我们的研究结果表明，RYBP通过调节减数分裂和精子活力在精子发生中发挥重要作用。
