Institute of Veterinary Clinical Science, School of Veterinary Medicine, College of Bio-Resources and Agriculture, National Taiwan University, Taipei City, Taiwan.
National Taiwan University Veterinary Hospital, College of Bio-Resources and Agriculture, National Taiwan University, Taipei City, Taiwan.
J Vet Intern Med. 2020 May;34(3):1222-1230. doi: 10.1111/jvim.15781. Epub 2020 Apr 23.
Soluble-type hemojuvelin in serum and urine has been shown to be a biomarker in humans for chronic kidney disease (CKD) and acute kidney injury (AKI). No similar research has been conducted on cats.
Urine hemojuvelin (u-hemojuvelin) can be used as a clinical indicator for cats with various renal diseases.
Eighteen healthy cats, 10 cats with AKI, 21 cats with acute-on-chronic kidney injury (ACKI), and 45 cats with CKD were enrolled.
The expression profile of u-hemojuvelin was assessed by Western blot analysis, whereas the u-hemojuvelin concentration was measured using an in-house sandwich ELISA. Each cat's u-hemojuvelin-to-creatinine ratio (UHCR) also was determined.
Significant differences were found in both u-hemojuvelin concentration and UHCR between the control cats and the other cats (AKI, CKD, ACKI). Both u-hemojuvelin and UHCR had high areas under the receiver operator curve (AUROC) for diagnoses of AKI (u-hemojuvelin, 0.885; UHCR, 0.982), CKD (hemojuvelin, 0.869; UHCR, 0.959), and ACKI (hemojuvelin, 0.910; UHCR, 1). Late stage (International Renal Interest Society, IRIS stages 3 and 4) CKD cats had significantly higher u-hemojuvelin concentration and UHCR than did early stage cats (IRIS stages 1 and 2). Both u-hemojuvelin and UHCR were significantly correlated with high blood urea nitrogen, plasma creatinine, and plasma phosphate concentrations and with low hematocrit (Hct), red blood cell (RBC) count, and plasma albumin concentration. The UHCR values were also significantly correlated with white blood cell count in blood.
Both u-hemojuvelin and UHCR potentially can serve as diagnostic indicators for a range of renal diseases in cats.
血清和尿液中的可溶性转铁蛋白受体已被证明是人类慢性肾脏病（CKD）和急性肾损伤（AKI）的生物标志物。猫科动物尚未进行类似的研究。
尿转铁蛋白（u-hemojuvelin）可用作各种肾脏疾病猫的临床指标。
纳入 18 只健康猫、10 只 AKI 猫、21 只急-慢肾损伤（ACKI）猫和 45 只 CKD 猫。
采用 Western blot 分析评估 u-hemojuvelin 的表达谱，采用内部夹心 ELISA 法测定 u-hemojuvelin 浓度。还测定了每只猫的 u-hemojuvelin 与肌酐比值（UHCR）。
在对照组猫和其他猫（AKI、CKD、ACKI）之间，u-hemojuvelin 浓度和 UHCR 均存在显著差异。u-hemojuvelin 和 UHCR 对 AKI（u-hemojuvelin，0.885；UHCR，0.982）、CKD（hemojuvelin，0.869；UHCR，0.959）和 ACKI（hemojuvelin，0.910；UHCR，1）的诊断均具有较高的受试者工作特征曲线下面积（AUROC）。晚期（国际肾脏学会，IRIS 分期 3 和 4）CKD 猫的 u-hemojuvelin 浓度和 UHCR 明显高于早期（IRIS 分期 1 和 2）猫。u-hemojuvelin 和 UHCR 均与高血尿素氮、血浆肌酐和血浆磷酸盐浓度以及低血细胞比容（Hct）、红细胞（RBC）计数和血浆白蛋白浓度显著相关。UHCR 值与血液中的白细胞计数也显著相关。
u-hemojuvelin 和 UHCR 均可能成为猫各种肾脏疾病的诊断指标。
