Department of Food Science and Technology, Pukyong National University, Busan 48513, Republic of Korea; University of Cuu Long, 1A Highway, Long Ho District, Vinh Long 85000, Viet Nam.
Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea.
Int J Biol Macromol. 2020 Aug 15;157:484-493. doi: 10.1016/j.ijbiomac.2020.04.113. Epub 2020 Apr 20.
In this study, pressurized liquid extraction (PLE) of polyphenolic-polysaccharide (PP) from Pseuderanthemum palatiferum (Nees) Radlk. leaves was carried out and compared with a conventional technique using 0.1 M sodium hydroxide. The extracts were purified according to the method reported previously to obtain PP conjugates which were further studied about chemical profiles and anticoagulant activity. Fourier-transform infrared spectroscopy (FTIR), UV-Vis, nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), and spectrophotometry analysis were used to characterize the selected PP conjugates. The results showed that PP conjugates comprised of carbohydrate, phenolic, and protein constituents with the yield ranged from 2.76% to 14.34%. Seven mono sugars containing in all conjugates were determined using high-performance liquid chromatography (HPLC), namely, arabinose, fucose, galactose, glucose, mannose, rhamnose, and xylose. PP conjugates obtained from PLE at 150 °C (PP-PLE5) exhibited better anticoagulant activity than those found at 200 °C and comparable to that of the conventional technique. On gel permeation chromatography, PP-PLE5 showed a broad molecular mass from 6 to 642 kDa. From the obtained results, PLE can be used as a green effective technique for the recovery of PP conjugate from P. palatiferum leaves.
本研究采用加压液体萃取（PLE）法从 Nees 叶 Palatiferum（假杜鹃）中提取多酚-多糖（PP），并与传统的 0.1 M 氢氧化钠法进行了比较。提取物根据先前报道的方法进行纯化，以获得 PP 缀合物，进一步研究其化学特征和抗凝活性。傅里叶变换红外光谱（FTIR）、紫外-可见光谱（UV-Vis）、核磁共振（NMR）、凝胶渗透色谱（GPC）和分光光度分析用于表征所选的 PP 缀合物。结果表明，PP 缀合物由碳水化合物、酚类和蛋白质组成，产率在 2.76%至 14.34%之间。使用高效液相色谱法（HPLC）测定了所有缀合物中含有的七种单糖，分别为阿拉伯糖、岩藻糖、半乳糖、葡萄糖、甘露糖、鼠李糖和木糖。在 150°C 下从 PLE 获得的 PP-PLE5 具有比在 200°C 下获得的更好的抗凝活性，与传统技术相当。在凝胶渗透色谱上，PP-PLE5 显示出 6 至 642 kDa 的宽分子量分布。从研究结果可以看出，PLE 可作为一种从 Palatiferum 叶中回收 PP 缀合物的绿色有效技术。
