Laboratory of Bio-Control and Molecular Biology, Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi Arabia.
Research and Consulting Institute, King Faisal University, Hofuf 31982, Al-Ahsa, Saudi Arabia.
Molecules. 2020 Apr 20;25(8):1900. doi: 10.3390/molecules25081900.
Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of through natural products. In this regard, in vitro tests were performed to evaluate the interaction of spores with multiple doses of 1-Chlorooctadecane (0.8, 1.6, 2.4, 3.2, and 4.0 mg/mL). Compatibility bioassay results evidenced from vegetative growth (77.7-84.40 mm), sporulation (5.50-7.30 × 10 spores/mL), and germination (96.70-98.20%), revealed that all the tested doses are compatible (biological index > 82) with the spores of . The impact of combined treatment of spores with 1-Chlorooctadecane in different proportions (Scheme I, II, III, and IV) compared to their sole application against was evaluated by concentration-mortality response bioassays. Results showed that all the combined treatments revealed high mortality compared to the sole application, which showed relatively slow mortality response over time. Toxicity recorded from Scheme IV combinations (80% 1-Chlorooctadecane: 20% Spores), exhibited strong synergistic interaction (joint toxicity = 713). Furthermore, potent interactions have overcome the host antioxidant defense at the final stage of infection by tremendously reducing catalase, and superoxide dismutase activities. These experiments demonstrated fungal-toxin joint synergistic interaction as a promising date palm dust mite management option.
椰枣粉螨是一种重要的害虫，严重侵害有价值的营养椰枣果实。为了寻找杀螨剂的替代品，评估了 EBCL 02049 孢子和 1-氯十八烷的联合作用作为通过天然产物防治 的潜在候选物。在这方面，进行了体外试验以评估 孢子与多种剂量的 1-氯十八烷（0.8、1.6、2.4、3.2 和 4.0 mg/mL）的相互作用。营养生长（77.7-84.40 mm）、孢子形成（5.50-7.30×10 个孢子/mL）和萌发（96.70-98.20%）的兼容性生物测定结果表明，所有测试剂量均与 的孢子相容（生物指数>82）。与单独应用相比，评估了孢子与 1-氯十八烷以不同比例（方案 I、II、III 和 IV）联合处理对 的影响。浓度-致死反应生物测定结果表明，与单独应用相比，所有联合处理均显示出更高的死亡率，而单独应用随着时间的推移死亡率反应相对较慢。方案 IV 组合（80% 1-氯十八烷：20% 孢子）记录的毒性表现出很强的协同相互作用（联合毒性=713）。此外，强大的相互作用克服了宿主抗氧化防御，在感染的最后阶段极大地降低了过氧化氢酶和超氧化物歧化酶的活性。这些实验证明了真菌毒素联合协同相互作用作为一种有前途的椰枣粉螨管理选择。
