Department of Stomatology, Affiliated Hospital of Qingdao University, No. 16 Jiangsu Road, Shinan District, Qingdao, 266003, China.
School of Stomatology of Qingdao University, Qingdao, 266003, China.
Mycopathologia. 2020 Jun;185(3):485-494. doi: 10.1007/s11046-020-00446-9. Epub 2020 Apr 23.
The aim of this research was to study the effects of different concentrations of magnesium oxide nanoparticles (MgO NPs) on the growth and key virulence factors of Candida albicans (C. albicans). The minimum inhibitory concentration (MIC) of MgO NPs against C. albicans was determined by the micro-broth dilution method. A time-kill curve of MgO NPs and C. albicans was established to investigate the ageing effect of MgO NPs on C. albicans. Crystal violet staining, the MTT assay, and inverted fluorescence microscopy were employed to determine the effects of MgO NPs on C. albicans adhesion, two-phase morphological transformation, biofilm biomass, and metabolic activity. The time-kill curve showed that MgO NPs had fungicidal and antifungal activity against C. albicans in a time- and concentration-dependent manner. Semi-quantitative crystal violet staining and MTT assays showed that MgO NPs significantly inhibited C. albicans biofilm formation and metabolic activity, and the difference was statistically significant (p < 0.001). Inverted fluorescence microscopy showed that MgO NPs could inhibit the formation of C. albicans biofilm hyphae. Adhesion experiments showed that MgO NPs significantly inhibited the initial adhesion of C. albicans (p < 0.001). This study demonstrates that MgO NPs can effectively inhibit the growth, initial adhesion, two-phase morphological transformation, and biofilm formation of C. albicans and is an antifungal candidate.
本研究旨在研究不同浓度氧化镁纳米粒子（MgO NPs）对白色念珠菌（C. albicans）生长和关键毒力因子的影响。采用微量肉汤稀释法测定 MgO NPs 对 C. albicans 的最小抑菌浓度（MIC）。建立 MgO NPs 和 C. albicans 的时程杀灭曲线，以研究 MgO NPs 对 C. albicans 的老化作用。采用结晶紫染色、MTT 检测和倒置荧光显微镜观察法，测定 MgO NPs 对 C. albicans 黏附、两相形态转化、生物膜生物量和代谢活性的影响。时程杀灭曲线显示，MgO NPs 对 C. albicans 具有时间和浓度依赖性的杀菌和抑菌活性。半定量结晶紫染色和 MTT 检测结果表明，MgO NPs 显著抑制 C. albicans 生物膜形成和代谢活性，差异具有统计学意义（p<0.001）。倒置荧光显微镜观察显示，MgO NPs 可抑制 C. albicans 生物膜菌丝的形成。黏附实验表明，MgO NPs 显著抑制 C. albicans 的初始黏附（p<0.001）。本研究表明，MgO NPs 能有效抑制 C. albicans 的生长、初始黏附、两相形态转化和生物膜形成，是一种有前途的抗真菌候选药物。
