子痫前期中胎盘 IL9 和 IL9R 的减少损害滋养细胞的增殖、侵袭和血管生成。
Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis.
机构信息
Obstetric department, Obstetrics and Gynecology Hospital of Fudan University , Shanghai, China.
Obstetric department, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases , Shanghai, China.
出版信息
Hypertens Pregnancy. 2020 Aug;39(3):228-235. doi: 10.1080/10641955.2020.1754852. Epub 2020 Apr 24.
OBJECTIVE
Our study aimed to investigate IL9 and IL9R expression in preeclampsia and assess their effects on trophoblast biological behaviors.
METHODS
IL9 and IL9R expression of placenta tissue were evaluated by immunochemistry and q-PCR. Using transwell, CCK-8, and tubule formation assays measured invasion, proliferation and angiogenesis of trophoblast with adding IL9 or anti-IL9R antibody.
RESULTS
IL9 and IL9R levels were significantly decreased in preeclampsia. IL9 improved trophoblast activities. Blocking IL9/IL9R resulted in decreased proliferation, invasion, and tube-formation capability of trophoblast.
CONCLUSIONS
IL9 and IL9R contribute to the pathogenesis of preeclampsia. IL9/IL9R signaling provides a new potential therapeutic target for preventing preeclampsia.
目的
本研究旨在探讨子痫前期中白细胞介素 9（IL9）及其受体（IL9R）的表达情况，并评估其对滋养细胞生物学行为的影响。
方法
采用免疫组化和 q-PCR 检测胎盘组织中 IL9 和 IL9R 的表达。通过 Transwell、CCK-8 和管腔形成实验，在加入 IL9 或抗 IL9R 抗体后，检测滋养细胞的侵袭、增殖和血管生成能力。
结果
子痫前期患者中 IL9 和 IL9R 的水平显著降低。IL9 可增强滋养细胞的活性。阻断 IL9/IL9R 会降低滋养细胞的增殖、侵袭和管腔形成能力。
结论
IL9 和 IL9R 参与了子痫前期的发病机制。IL9/IL9R 信号通路为预防子痫前期提供了一个新的潜在治疗靶点。
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