环状 RNA（circRNA）PAPPA2 通过调控 miR-942/miR-5006-3p 在子痫前期发病机制中发挥作用。
CircPAPPA2 plays a role in preeclampsia pathogenesis via regulation of the miR-942/miR-5006-3p.
机构信息
Department of Gynaecology and Obstetrics, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
Department of Hepatopancreatobiliary Surgery, Hengyang Medical School, The First Affiliated Hospital, University of South China. NO.69, Chuanshan Road, Hengyang, 421001, Hunan, China.
出版信息
BMC Pregnancy Childbirth. 2024 Jun 7;24(1):414. doi: 10.1186/s12884-024-06560-6.
CircRNAs are a class of endogenous non-coding RNAs implicated in the pathogenesis of many pregnancy related diseases, one of which is pre-eclampsia (PE). This study aims to investigate the role of CircPAPPA2 (circbase ID: hsa_circ_0015382) in regulating the migration and invasion of trophoblast cells. RNA sequencing was used to identify the differentially expressed circRNAs in placenta of PE and normal pregnant women. Quantitative polymerase chain reaction (qRT-PCR) was used to verify the expression of circPAPPA2 and two miRNAs (miR-942-5p, 5006-3p) in placenta of PE and normal pregnant women. CCK8 and transwell experiments were performed to assess the function of circPAPPA2 in PE development.The interaction between circPAPPA2 and miR-942-5p/miR-5006-3p was verified by dual-luciferase reporter assay. Finally, bioinformatics analyzed with gene ontology, Kyoto Encyclopedia of the target genes. The results showed that the expression of circPAPPA2 was increased in placenta of PE pregnant women. Also, circPAPPA2 impedes trophoblasts cell proliferation and invasion. Moreover, the expression of circPAPPA2 was positively correlated with systolic blood pressure and urine protein. In addition, circPAPPA2 serves as a sponge of miR-942-5p and miR-5006-3p. In conclusion, CircPAPPA2 regulates trophoblasts cell proliferation and invasion by mediating the miR-942/miR-5006-3p.
环状 RNA 是一类内源性非编码 RNA，参与多种妊娠相关疾病的发病机制，其中之一是子痫前期 (PE)。本研究旨在探讨 CircPAPPA2（circbase ID：hsa_circ_0015382）在调节滋养细胞迁移和侵袭中的作用。使用 RNA 测序鉴定 PE 和正常孕妇胎盘差异表达的 circRNAs。定量聚合酶链反应 (qRT-PCR) 用于验证 PE 和正常孕妇胎盘 circPAPPA2 和两种 miRNA（miR-942-5p、5006-3p）的表达。CCK8 和 Transwell 实验评估 circPAPPA2 在 PE 发展中的作用。通过双荧光素酶报告实验验证 circPAPPA2 与 miR-942-5p/miR-5006-3p 的相互作用。最后，对靶基因进行基因本体和京都基因与基因组百科全书的生物信息学分析。结果表明，PE 孕妇胎盘 circPAPPA2 表达增加。此外，circPAPPA2 抑制滋养细胞增殖和侵袭。而且，circPAPPA2 的表达与收缩压和尿蛋白呈正相关。此外，circPAPPA2 作为 miR-942-5p 和 miR-5006-3p 的海绵。总之，CircPAPPA2 通过调节 miR-942/miR-5006-3p 来调节滋养细胞的增殖和侵袭。
Zotero 插件