Department of Interventional Therapy, The Third Affiliated Hospital of Wenzhou Medical University , Wenzhou, Zhejiang, China.
Department of Gastroenterology, The Third Affiliated Hospital of Wenzhou Medical University , Wenzhou, Zhejiang, China.
Cancer Biol Ther. 2020 Jul 2;21(7):604-614. doi: 10.1080/15384047.2020.1738908. Epub 2020 Apr 24.
Long noncoding RNAs (lncRNAs) have been reported to play a significant role in the occurrence and progression of tumors. In different tumors, they can either act as an oncogene or tumor suppressor via modulating various target mRNAs. OIP5-AS1 belongs to lncRNA family. It has been reported to be involved in the tumorigenesis of some cancers, such as bladder cancer, gastric cancer, and multiple myeloma. However, the role it plays in hepatocellular carcinoma (HCC) remains unclear. This study aims to explore the inherent mechanism of lncRNA OIP5-AS1 in HCC. In the first place, qRT-PCR found that OIP5-AS1 and VEGFA expressions were significantly increased while miR-3163 was obviously reduced in HCC cells and tissues. Next, a series of functional experiments found that knockdown of OIP5-AS1 suppressed HCC cell proliferation, migration and angiogenesis abilities while promoting cell apoptosis simultaneously. Last but not least, miR-3163 inhibition or VEGFA overexpression can reverse the anti-tumor effect of OIP5-AS1. In summary, OIP5-AS1 affects HCC proliferation, metastasis, and angiogenesis in HCC by regulating VEGFA expression through sponging miR-3163.
长链非编码 RNA（lncRNA）已被报道在肿瘤的发生和发展中发挥重要作用。在不同的肿瘤中，它们可以通过调节各种靶 mRNA 来充当癌基因或肿瘤抑制因子。OIP5-AS1 属于 lncRNA 家族。据报道，它参与了一些癌症的肿瘤发生，如膀胱癌、胃癌和多发性骨髓瘤。然而，它在肝细胞癌（HCC）中所起的作用尚不清楚。本研究旨在探讨 lncRNA OIP5-AS1 在 HCC 中的内在机制。首先，qRT-PCR 发现 OIP5-AS1 和 VEGFA 的表达在 HCC 细胞和组织中显著增加，而 miR-3163 的表达明显降低。接下来，一系列功能实验发现，敲低 OIP5-AS1 同时抑制 HCC 细胞增殖、迁移和血管生成能力，同时促进细胞凋亡。最后但并非最不重要的是，miR-3163 抑制或 VEGFA 过表达可以逆转 OIP5-AS1 的抗肿瘤作用。总之，OIP5-AS1 通过海绵吸附 miR-3163 来调节 VEGFA 的表达，从而影响 HCC 增殖、转移和血管生成。
