大球体多分散悬浮液的动态光散射。分离的分泌颗粒的表征。
Dynamic light scattering from polydisperse suspensions of large spheres. Characterization of isolated secretory granules.
作者信息
Fujime S, Takasaki-Ohsita M, Miyamoto S
机构信息
Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo, Japan.
出版信息
Biophys J. 1988 Dec;54(6):1179-83. doi: 10.1016/S0006-3495(88)83054-6.
Dynamic light scattering is useful in determining the diameter of submicrometer particles in suspension. When both static scattering intensity P(K) and apparent diffusion coefficient D can be measured in a wide range of the length of the scattering vector K, it is possible to determine the number-average diameter dn and sharpness in size distribution of spheres. We derived approximate, but very simple, expressions for mean value of P(K) and mean value of D/D(dn) applicable to very large spheres for which the so-called Rayleigh-Debye condition is perturbed, where mean value of ... stands for the size average. These approximate expressions were compared with the numerical results based on the Mie scattering theory. Experimental results for isolated secretory granules, zymogen (dn approximately 800 nm) and chromaffin (dn approximately 400 nm) granules, were analyzed by use of the present formulation, and the dispersion in size distribution, sigma/dn = [(the mean of d2)/d2n - 1]1/2, was found to be about 0.2 for both types of granules.
动态光散射在确定悬浮液中亚微米级颗粒的直径方面很有用。当在散射矢量K的较宽范围内都能测量静态散射强度P(K)和表观扩散系数D时，就有可能确定球体的数均直径dn和尺寸分布的尖锐度。我们推导了适用于所谓瑞利-德拜条件受到扰动的非常大球体的P(K)平均值和D/D(dn)平均值的近似但非常简单的表达式，其中……的平均值表示尺寸平均值。将这些近似表达式与基于米氏散射理论的数值结果进行了比较。利用本公式分析了分离的分泌颗粒、酶原颗粒（dn约为800nm）和嗜铬颗粒（dn约为400nm）的实验结果，发现这两种颗粒的尺寸分布离散度sigma/dn = [(d2的平均值)/d2n - 1]1/2约为0.2。
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