来自直径为亚微米级的薄旋转椭球壳多分散悬浮液的动态光散射。

Dynamic light scattering from polydisperse suspensions of thin ellipsoidal shells of revolution with submicron diameters.

作者信息

Fujime S, Takasaki-Ohsita M, Miyamoto S

机构信息

Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.

出版信息

Biophys J. 1988 Apr;53(4):497-503. doi: 10.1016/S0006-3495(88)83129-1.

DOI:10.1016/S0006-3495(88)83129-1

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1330223/

Abstract

Osmotic swelling of membrane vesicles has been studied in combination with dynamic light scattering, to obtain information about the elastic properties of biomembranes. In such studies, there arise some technical problems specific to dynamic light scattering, which include the effects on the light-scattering results of the size distribution and nonsphericity of the vesicles with submicron sizes. Even for highly monodisperse suspensions of spherical vesicles (sigma/dn = [(the mean of d2)/d2n-1]1/2 = 0.1; dn being the number-average diameter of vesicles), the average diameter d obtained from dynamic light scattering is shown to be strongly dependent on dnK, where K is the length of the scattering vector. This is solely due to the shell structure of the vesicles. For ellipsoidal vesicles, another complication appears which is due to the rotational motion of ellipsoids.

摘要

已结合动态光散射研究了膜泡的渗透肿胀，以获取有关生物膜弹性特性的信息。在这类研究中，出现了一些动态光散射特有的技术问题，其中包括亚微米尺寸的膜泡的尺寸分布和非球形对光散射结果的影响。即使对于球形膜泡的高度单分散悬浮液（σ/dn = [(d2的平均值)/d2n - 1]1/2 = 0.1；dn为膜泡的数均直径），从动态光散射获得的平均直径d也显示出强烈依赖于dnK，其中K是散射矢量的长度。这完全是由于膜泡的壳结构。对于椭圆形膜泡，出现了另一个复杂情况，这是由于椭球体的旋转运动所致。

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