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Change in membrane elastic modulus on activation of glucose transport system of brush border membrane vesicles studied by osmotic swelling and dynamic light scattering.通过渗透肿胀和动态光散射研究刷状缘膜囊泡葡萄糖转运系统激活时膜弹性模量的变化。
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本文引用的文献

1
Dynamic light scattering from dilute suspensions of thin discs and thin rods as limiting forms of cylinder, ellipsoid and ellipsoidal shell of revolution.作为圆柱、椭球体和旋转椭球壳的极限形式，来自薄圆盘和细棒的稀悬浮液的动态光散射。
Biophys Chem. 1985 Nov;23(1-2):1-13. doi: 10.1016/0301-4622(85)80059-4.
2
Purification of brush border membrane by thiocyanate treatment.通过硫氰酸盐处理纯化刷状缘膜。
Anal Biochem. 1983 Jun;131(2):447-52. doi: 10.1016/0003-2697(83)90197-5.
3
Elasticity of synthetic phospholipid vesicles and submitochondrial particles during osmotic swelling.渗透肿胀过程中合成磷脂囊泡和亚线粒体颗粒的弹性
Biochemistry. 1986 Dec 16;25(25):8220-9. doi: 10.1021/bi00373a015.
4
Change in membrane elastic modulus on activation of glucose transport system of brush border membrane vesicles studied by osmotic swelling and dynamic light scattering.通过渗透肿胀和动态光散射研究刷状缘膜囊泡葡萄糖转运系统激活时膜弹性模量的变化。
Biophys J. 1988 Apr;53(4):505-12. doi: 10.1016/S0006-3495(88)83130-8.
5
Osmotically-induced and photo-induced deformations of disk membranes.盘状膜的渗透诱导变形和光诱导变形。
Biochim Biophys Acta. 1977 Dec 15;471(3):436-52. doi: 10.1016/0005-2736(77)90048-7.

来自直径为亚微米级的薄旋转椭球壳多分散悬浮液的动态光散射。

Dynamic light scattering from polydisperse suspensions of thin ellipsoidal shells of revolution with submicron diameters.

作者信息

Fujime S, Takasaki-Ohsita M, Miyamoto S

机构信息

Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.

出版信息

Biophys J. 1988 Apr;53(4):497-503. doi: 10.1016/S0006-3495(88)83129-1.

DOI:10.1016/S0006-3495(88)83129-1
PMID:3382709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1330223/
Abstract

Osmotic swelling of membrane vesicles has been studied in combination with dynamic light scattering, to obtain information about the elastic properties of biomembranes. In such studies, there arise some technical problems specific to dynamic light scattering, which include the effects on the light-scattering results of the size distribution and nonsphericity of the vesicles with submicron sizes. Even for highly monodisperse suspensions of spherical vesicles (sigma/dn = [(the mean of d2)/d2n-1]1/2 = 0.1; dn being the number-average diameter of vesicles), the average diameter d obtained from dynamic light scattering is shown to be strongly dependent on dnK, where K is the length of the scattering vector. This is solely due to the shell structure of the vesicles. For ellipsoidal vesicles, another complication appears which is due to the rotational motion of ellipsoids.

摘要

已结合动态光散射研究了膜泡的渗透肿胀，以获取有关生物膜弹性特性的信息。在这类研究中，出现了一些动态光散射特有的技术问题，其中包括亚微米尺寸的膜泡的尺寸分布和非球形对光散射结果的影响。即使对于球形膜泡的高度单分散悬浮液（σ/dn = [(d2的平均值)/d2n - 1]1/2 = 0.1；dn为膜泡的数均直径），从动态光散射获得的平均直径d也显示出强烈依赖于dnK，其中K是散射矢量的长度。这完全是由于膜泡的壳结构。对于椭圆形膜泡，出现了另一个复杂情况，这是由于椭球体的旋转运动所致。