Rosenheck K, Lindner P, Pecht I
J Membr Biol. 1975;20(1-2):1-12. doi: 10.1007/BF01870624.
Electric field pulses (in the 5 to 25 kV/cm range) were found to cause a transient increase in the intensity of scattered light (wavelength equals 369 nm, scattering angle equals 90 degrees) from aqueous sucrose suspensions of chromaffin granules. Similar observations were made with the membranes of osmotically lysed chromaffin granules. Under the same experimental conditions the degree of polarization of the scattered light changed only very slightly. The fluorescence of the hydrophobic probe diphenyl-hexatriene, incorporated into the membrane of intact chromaffin granules, showed similar transient changes in the intensity. The calculated relaxation times for these changes in optical properties were approximately 150 musec for the rising phase, and approximately 1 msec for the early stage of the decay. A further relaxation time of about 30 msec was also observed by using this probe. Essentially, all of these signals originated from the granule membrane, and could be attributed to rather small changes in particle size, membrane thickness or refractive index. Moreover, these signals were found to be completely reversible. Catecholamine release from intact granules, pulsed at voltages of 25 kV/cm, occurs already during the first few milliseconds of the transient membrane change.
电场脉冲（5至25 kV/cm范围）被发现会导致嗜铬粒蛋白颗粒的蔗糖水悬浮液散射光强度（波长等于369 nm，散射角等于90度）出现短暂增加。对渗透性裂解的嗜铬粒蛋白颗粒的膜也进行了类似观察。在相同实验条件下，散射光的偏振度仅发生非常轻微的变化。完整嗜铬粒蛋白颗粒膜中掺入的疏水探针二苯基己三烯的荧光强度也出现了类似的短暂变化。这些光学性质变化的计算弛豫时间，上升阶段约为150微秒，衰减早期约为1毫秒。使用该探针还观察到约30毫秒的进一步弛豫时间。基本上，所有这些信号都源自颗粒膜，并且可归因于颗粒大小、膜厚度或折射率的相当小的变化。此外，这些信号被发现是完全可逆的。在25 kV/cm电压下施加脉冲时，完整颗粒中的儿茶酚胺释放已经在膜瞬态变化的最初几毫秒内发生。
