Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt.
Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia.
Bull Environ Contam Toxicol. 2020 Jun;104(6):858-863. doi: 10.1007/s00128-020-02856-3. Epub 2020 Apr 24.
To study the genotoxic impacts of Sabal discharges, three sites around Sabal drain were selected and compared to a reference site (site1). Site2 was at the southern part of the main canal, site3 was at the main canal outlet, and site4 at the northern part of the main canal. Compared to the reference fish, the recorded micronuclei and other nuclear anomalies showed marked (p < 0.05) increases with different frequencies in the studied sites. The induction of nuclear deformations was as following site3 > site4 > site2 > site1. The analysis of comet assay data showed that the DNA damage (based on the percentage of tail DNA) was significantly increased and the levels of damage were associated with the distance from the main discharge point. Moreover, DNA damages showed variable percentages among the studied tissues. The gills and liver tissues collected from site3 showed the highest DNA damage compared with low muscular DNA damage.
为了研究萨巴尔排水的遗传毒性影响，选择了萨巴尔排水周围的三个地点，并与一个参考地点（site1）进行了比较。site2 位于主运河的南部，site3 位于主运河出口处，site4 位于主运河的北部。与参考鱼类相比，在所研究的地点记录的微核和其他核异常以不同的频率显著增加（p<0.05）。核变形的诱导如下：site3>site4>site2>site1。彗星试验数据分析表明，DNA 损伤（基于尾 DNA 的百分比）显著增加，并且损伤水平与离主排放点的距离有关。此外，在所研究的组织中，DNA 损伤的百分比不同。与低肌肉 DNA 损伤相比，从 site3 收集的鳃和肝脏组织显示出最高的 DNA 损伤。
