Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM). C/Nicolás Cabrera, 9, Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain.
Food Funct. 2020 May 1;11(5):4081-4089. doi: 10.1039/d0fo00236d. Epub 2020 Apr 25.
The degree of digestion, modulated by rat small intestinal extract on different functional fibers was investigated. In general, inulin-type fructans and fructooligosaccharides were the most resistant to the enzymatic digestion. Results evidenced the high-resistance of fructosyl-fructose bonds. This fits well with the concept of prebiotic carbohydrates. However, the mixture of melibiose, manninotriose and verbascotetraose (α-GOS) from peas, with a considerably lower molecular weight (0.6 kDa) than the fructans studied, were highly digested (61.2%). Interestingly, the Gal-(1 → 6)-Gal bonds present into the manninotriose and verbascotetraose were more prone to be hydrolyzed than Gal-(1 → 6)-Glc (melibiose). However, when melibiose was the only disaccharide present in the reaction mixture, the hydrolysis was also high (67.7%). The use of small intestinal enzymatic preparations is a realistic approximation to evaluate the digestion of different carbohydrates, thus, showing that recognized non-digestible carbohydrates can also be partially digested.
研究了大鼠小肠提取物对不同功能纤维的消化程度的调节。一般来说，菊粉型果聚糖和低聚果糖的抗酶消化能力最强。结果表明，果糖基-果糖键具有很高的抗性。这与前生物碳水化合物的概念非常吻合。然而，豌豆中含有低分子量（0.6 kDa）的混合蜜二糖、甘露三糖和毛蕊花糖（α-GOS），其消化程度很高（61.2%）。有趣的是，甘露三糖和毛蕊花糖中存在的 Gal-(1 → 6)-Gal 键比 Gal-(1 → 6)-Glc（蜜二糖）更容易被水解。然而，当反应混合物中只有蜜二糖作为唯一的二糖存在时，水解率也很高（67.7%）。使用小肠酶制剂是评估不同碳水化合物消化程度的一种现实方法，表明公认的不可消化碳水化合物也可以部分消化。
