Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Lab Chip. 2020 May 5;20(9):1621-1627. doi: 10.1039/d0lc00304b.
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
快速、灵敏和特异性检测和报告传染性病原体对于患者管理和疫情监测非常重要。我们展示了一种即时检测系统，该系统与智能手机集成，用于从鼻拭子介质中检测活病毒，使用马呼吸道传染病检测 panel 作为相应人类疾病（如 COVID-19）的模型系统。五种病原体的特异性核酸序列在微流控芯片上通过环介导等温扩增进行扩增，并在反应结束时通过智能手机进行检测。我们的系统可以正确诊断含有病原体的马鼻拭子样本，检测限与传统实验室检测方法聚合酶链反应相当，结果在 30 分钟内得出。
