Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.
Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Protein Pept Lett. 2020;27(10):1007-1021. doi: 10.2174/1871530320666200425203636.
Drug-protein complexes is one of the crucial factors when analyzing the pharmacokinetics and pharmacodynamics of a drug because they can affect the excretion, distribution, metabolism and interaction with target tissues.
The aim of this study was to investigate the interaction of human hemoglobin (Hb) and angiotensin I converting enzyme inhibitory peptide (ACEIP) in the absence and presence of different- frequency electromagnetic fields (EMF).
Various spectroscopic methods like fluorescence spectroscopy, ultraviolet, circular dichroism and conductometry techniques were applied to investigate Hb-ACEIP interaction in the absence and presence of EMF.
The presented spectroscopic studies indicated that EMF changed the interaction between Hb and ACEIP. The a-helix content of Hb decreased upon binding to ACEIP and conductivity of the solution enhanced upon binding. Based on Stern-Volmer equations, it could be stated that the Hb-ACEIP affinity was higher in the presence of EMF.
It can be concluded that for patients who use the drug to control blood pressure, a low-frequency electromagnetic field would have a positive effect on the uptake of the drug.
药物-蛋白质复合物是分析药物药代动力学和药效动力学的关键因素之一，因为它们会影响药物的排泄、分布、代谢和与靶组织的相互作用。
本研究旨在研究在不存在和存在不同频率电磁场（EMF）的情况下，人血红蛋白（Hb）与血管紧张素转化酶抑制肽（ACEIP）的相互作用。
应用荧光光谱法、紫外光谱法、圆二色性和电导法等多种光谱方法研究 EMF 存在和不存在时 Hb-ACEIP 相互作用。
所进行的光谱研究表明，EMF 改变了 Hb 与 ACEIP 之间的相互作用。Hb 与 ACEIP 结合后，α-螺旋结构含量降低，溶液电导率增强。根据 Stern-Volmer 方程，可以得出结论，在存在 EMF 的情况下，Hb-ACEIP 的亲和力更高。
可以得出结论，对于使用药物控制血压的患者，低频电磁场会对药物的吸收产生积极影响。
