大鼠视交叉上核内血管活性肠肽和肽组氨酸异亮氨酸免疫反应性及mRNA的定位

Localization of vasoactive intestinal peptide and peptide histidine isoleucine immunoreactivity and mRNA within the rat suprachiasmatic nucleus.

作者信息

Stopa E G, Minamitani N, Jonassen J A, King J C, Wolfe H, Mobtaker H, Albers H E

机构信息

Department of Pathology, Tufts University School of Medicine, Boston, MA.

出版信息

Brain Res. 1988 Dec;464(4):319-25. doi: 10.1016/0169-328x(88)90041-1.

DOI:10.1016/0169-328x(88)90041-1

PMID:3233491

Abstract

The distribution of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) mRNA within the suprachiasmatic nucleus (SCN) of rats was evaluated by immunocytochemistry and in situ hybridization. The pattern of VIP and PHI immunoreactivity corresponded closely to the distribution of VIP/PHI mRNA within the ventrolateral SCN. Clear hybridization signal was observed within the SCN of rats killed 5 h after light onset and in rats killed 2 h after the onset of the dark phase of the light-dark cycle. Visual examination of the grain density within the autoradiographs suggested that VIP/PHI mRNA may occur in higher concentrations shortly after the onset of darkness than 5 h after the onset of the light phase.

摘要

通过免疫细胞化学和原位杂交技术评估了大鼠视交叉上核（SCN）内血管活性肠肽（VIP）和肽组氨酸异亮氨酸（PHI）mRNA的分布。VIP和PHI免疫反应性模式与腹外侧SCN内VIP/PHI mRNA的分布密切对应。在光照开始后5小时处死的大鼠以及在明暗周期黑暗阶段开始后2小时处死的大鼠的SCN内观察到清晰的杂交信号。对放射自显影片内颗粒密度的目视检查表明，VIP/PHI mRNA在黑暗开始后不久的浓度可能高于光照阶段开始后5小时的浓度。