Okamoto S, Okamura H, Miyake M, Takahashi Y, Takagi S, Akagi Y, Fukui K, Okamoto H, Ibata Y
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Japan.
Histochemistry. 1991;95(5):525-8. doi: 10.1007/BF00315750.
We detected a diurnal change of vasoactive intestinal peptide (VIP) messenger RNA (mRNA) in the rat suprachiasmatic nucleus (SCN) using the in situ hybridization technique combined with computed image analysis. The amount of VIP mRNA was greatest at 02.00 h, the next largest level was seen at 20.00 h during the dark phase and smallest at 14.00 h during the light phase. We also confirmed that the onset of the fluctuation of mRNA occurred 2-6 h prior to the diurnal variation of the content of VIP-like immunoreactivity by semiquantitative immunocytochemistry. These findings suggest that light stimulus from the retina is a primarily influence on the transcription of VIP mRNA and induces a diurnal variation of VIP synthesis.
我们采用原位杂交技术结合计算机图像分析，检测了大鼠视交叉上核（SCN）中血管活性肠肽（VIP）信使核糖核酸（mRNA）的昼夜变化。VIP mRNA的量在凌晨2点时最多，在黑暗期的晚上8点时处于次高水平，而在光照期的下午2点时最少。我们还通过半定量免疫细胞化学证实，mRNA波动的起始时间比VIP样免疫反应性含量的昼夜变化提前2至6小时。这些发现表明，来自视网膜的光刺激是对VIP mRNA转录的主要影响因素，并诱导了VIP合成的昼夜变化。
