New South Wales Department of Primary Industries, Port Stephens Fisheries Institute, Taylors Beach, Australia.
Vet Med Sci. 2020 Aug;6(3):579-586. doi: 10.1002/vms3.272. Epub 2020 Apr 26.
The paired lateral abdominal veins (LAV) provide alternative venipuncture sites in grey nurse sharks (Carcharias taurus, Rafinesque 1810) and their efficacy was assessed using morphometrics, necropsies, ultrasound-guided blood sampling and by comparing serum biochemistry between the LAV and caudal vein (CV) with values from the latter published previously. The mean length of the CV was 2.8% of total length (TL) whereas each LAV was 22.4% of TL and, when combined, was approximately 16 times longer than the CV. The mean tissue thickness overlying each LAV increased significantly (p < .001) with increasing TL and ranged from 3.5 to 33.8 mm in the smallest to largest shark. The mean internal diameter of the paired LAV also increased significantly (p < .001) with increasing TL and was equal to or exceeded the CV. Experienced SCUBA divers captured 56 free-living grey nurse sharks and 46 healthy animals were sampled for blood from the LAV and CV with minimal risk to the animals or staff. Venipuncture of the LAV (n = 16) was easily accomplished using ultrasound guidance with a 38 mm/18-gauge needle, whereas standard methods were used with the CV (n = 30). Serum biochemistry was compared (t-tests) and none of the biochemical analytes differed significantly between the LAV and CV. The paired LAV produced representative blood samples and could also be used for fluid therapy and/or intravenous anaesthesia as has been done in other sharks. It is recommended that veterinary/husbandry staff familiarize themselves with the paired LAV and consider their use in the future.
成对的侧腹静脉（LAV）为灰护士鲨（Carcharias taurus，Rafinesque 1810）提供了替代的静脉穿刺部位，通过形态测量学、解剖、超声引导下的采血以及将 LAV 和尾静脉（CV）的血清生化指标与之前发表的后者进行比较，评估了其功效。CV 的平均长度为总长度（TL）的 2.8%，而每个 LAV 占 TL 的 22.4%，两者加起来的长度大约是 CV 的 16 倍。每个 LAV 上方的组织厚度与 TL 呈显著正相关（p<0.001），最小的鲨鱼为 3.5 毫米，最大的鲨鱼为 33.8 毫米。成对 LAV 的平均内径也随 TL 的增加而显著增加（p<0.001），并与 CV 相等或超过 CV。经验丰富的水肺潜水员捕获了 56 条自由生活的灰护士鲨，其中 46 条健康的鲨鱼从 LAV 和 CV 采血，对动物或工作人员的风险最小。使用超声引导，将 38 毫米/18 号针很容易对 LAV（n=16）进行静脉穿刺，而 CV（n=30）则使用标准方法。对血清生化指标进行了比较（t 检验），LAV 和 CV 之间没有一个生化指标有显著差异。成对的 LAV 产生了有代表性的血液样本，也可以用于液体治疗和/或静脉麻醉，这在其他鲨鱼中已经得到了应用。建议兽医/养殖人员熟悉成对的 LAV，并考虑在未来使用。
