Naples Lisa M, Mylniczenko Natalie D, Zachariah Trevor T, Wilborn Rachel E, Young Forrest A
John G. Shedd Aquarium, 1200 Lake Shore Dr, Chicago, IL 60605, USA.
J Am Vet Med Assoc. 2012 Jul 1;241(1):117-25. doi: 10.2460/javma.241.1.117.
To establish reference ranges for critical care blood values measured in wild and aquarium-housed elasmobranchs by use of a point-of-care (POC) blood analyzer and to compare values on the basis of species category (pelagic, benthic, or intermediate) and phlebotomy site.
Cross-sectional study.
66 wild and 89 aquarium-housed elasmobranchs (sharks and rays).
Aquarium-housed elasmobranchs were anesthetized for sample collection; wild elasmobranchs were caught via hook and line fishing, manually restrained for sample collection, and released. Blood was collected from 2 sites/fish (dorsal sinus region and tail vasculature) and analyzed with the POC analyzer. Reference values of critical care blood analytes were calculated for species most represented in each population. Values were compared on the basis of species categorization (pelagic, intermediate, or benthic) and collection site.
Oxygen saturation and circulating concentrations of lactate and glucose were significantly different among aquarium-housed pelagic, intermediate, and benthic species. Lactate concentration was significantly different among these categories in wild elasmobranchs. Significant differences were detected between samples from the 2 collection sites for all blood analytes. In both study populations, pH and lactate values were infrequently < 7.2 or > 5 mmol/L, respectively.
Brevity of handling or chemical restraint may have reduced secondary stress responses in fish because extreme variations in blood analyte values were infrequent. Sample collection site, species categorization, acclimation to handling, and restraint technique should be considered when assessing values obtained with the POC analyzer used in this study for blood analytes and immediate metabolic status in elasmobranchs.
使用即时检验（POC）血液分析仪建立野生及养殖在水族箱中的板鳃亚纲动物的重症监护血液值参考范围，并根据物种类别（远洋、底栖或中间型）和采血部位比较这些值。
横断面研究。
66只野生及89只养殖在水族箱中的板鳃亚纲动物（鲨鱼和鳐鱼）。
对养殖在水族箱中的板鳃亚纲动物进行麻醉以采集样本；野生板鳃亚纲动物通过钩钓捕获，手动固定以采集样本，然后放生。从每条鱼的2个部位（背窦区域和尾部血管系统）采集血液，并用POC分析仪进行分析。计算每个群体中最具代表性物种的重症监护血液分析物参考值。根据物种分类（远洋、中间型或底栖）和采集部位比较这些值。
养殖的远洋、中间型和底栖物种的血氧饱和度以及乳酸和葡萄糖的循环浓度存在显著差异。野生板鳃亚纲动物中，这些类别之间的乳酸浓度存在显著差异。所有血液分析物在两个采集部位的样本之间均检测到显著差异。在两个研究群体中，pH值和乳酸值分别很少低于7.2或高于5 mmol/L。
处理或化学约束的短暂性可能减少了鱼类的继发性应激反应，因为血液分析物值的极端变化很少见。在评估本研究中用于板鳃亚纲动物血液分析物和即时代谢状态的POC分析仪所获得的值时，应考虑样本采集部位、物种分类、对处理的适应程度和约束技术。
