Graduate School of Engineering, Osaka Prefecture University, Sakai, 599-8531, Japan.
Graduate School of Humanity and Sustainable System Sciences, Osaka Prefecture University, Sakai, 599-8531, Japan.
Anal Sci. 2020 Sep 10;36(9):1091-1097. doi: 10.2116/analsci.20P073. Epub 2020 Apr 24.
Roselle seeds, a waste biomass of the roselle calyx processing industry, were utilized to recover valuable compounds of oil, vitamin E, and water-soluble saccharides. Firstly, ultrasound-assisted extraction (UAE) and conventional stirring extraction were conducted for saccharide extraction, and the advantage of UAE was confirmed. Secondly, oil, vitamin E, and saccharides extracted from Vietnamese roselle seeds by UAE were analyzed for the first time. Oil of tri-, di-, and mono-glycerides, fatty acids of linoleic-, oleic-, palmitic-, and stearic-acids, vitamin E of γ- and α-tocopherol, and saccharides of sucrose, raffinose, stachyose, etc. were identified, and the amounts of these components were compared with those in other country's roselle seeds. Thirdly, cascade extraction of oil, vitamin E, and saccharides by UAE was investigated with solvents of hexane, hexane:ethyl acetate binary solvent, and water. The results indicated that the order of using solvents was very important for high and selective extraction: the best order to recover oil (almost 100%), vitamin E (95.7%), and saccharides (86.2%) was hexane, and then water.
玫瑰茄籽是玫瑰茄花萼加工业的一种废料生物质，可用于回收有价值的油、维生素 E 和水溶性糖。首先，进行了超声波辅助提取（UAE）和常规搅拌提取以提取糖，证实了 UAE 的优势。其次，首次分析了通过 UAE 从越南玫瑰茄籽中提取的油、维生素 E 和糖。鉴定了三酰基、二酰基和单甘油脂、亚油酸、油酸、棕榈酸和硬脂酸脂肪酸、γ-和α-生育酚维生素 E 以及蔗糖、棉子糖、水苏糖等糖，并且比较了这些成分的含量与其他国家的玫瑰茄种子中的含量。第三，用己烷、己烷-乙酸乙酯二元溶剂和水考察了 UAE 对油、维生素 E 和糖的级联提取。结果表明，溶剂的使用顺序对高选择性提取非常重要：回收油（几乎 100%）、维生素 E（95.7%）和糖（86.2%）的最佳顺序是先用己烷，然后用水。
