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用黄头病毒基因探针原位检测澳大利亚鳃相关病毒。

In situ detection of Australian gill-associated virus with a yellow head virus gene probe.

作者信息

Tang Kathy F-J, Spann Kirsten M, Owens Leigh, Lightner Donald V

机构信息

Department of Veterinary Science and Microbiology, University of Arizona, Tucson, AZ 85721, USA.

Co-operative Research Centre for Aquaculture, CSIRO Livestock Industries, 120 Meiers Road, Indooroopilly 4068, Australia.

出版信息

Aquaculture. 2002 Feb 28;205(1):1-5. doi: 10.1016/S0044-8486(01)00666-4. Epub 2002 Jan 29.

DOI:10.1016/S0044-8486(01)00666-4
PMID:32336809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7172130/
Abstract

A digoxigenin-labeled gene probe for yellow head virus (YHV) was used to detect gill-associated virus (GAV) in from Australia via in situ hybridization. In GAV-infected shrimp, positively reacting tissues included: lymphoid organ, gills, antennal gland, and cuticular epithelium of the stomach. This demonstrates that a YHV probe can be used as a diagnostic tool for GAV and supports previous suggestions that these two viruses are closely related.

摘要

使用地高辛配体标记的对虾黄头病毒(YHV)基因探针,通过原位杂交检测来自澳大利亚的虾中的鳃相关病毒(GAV)。在感染GAV的对虾中,呈阳性反应的组织包括:淋巴器官、鳃、触角腺和胃的表皮上皮。这表明YHV探针可作为GAV的诊断工具,并支持先前关于这两种病毒密切相关的推测。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e8/7172130/2baa711c8247/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e8/7172130/f409f3f4af15/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e8/7172130/2baa711c8247/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e8/7172130/f409f3f4af15/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87e8/7172130/2baa711c8247/gr2.jpg

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本文引用的文献

1
Gill-associated virus of Penaeus monodon prawns: an invertebrate virus with ORF1a and ORF1b genes related to arteri- and coronaviruses.斑节对虾鳃相关病毒:一种具有与动脉炎病毒和冠状病毒相关的ORF1a和ORF1b基因的无脊椎动物病毒。
J Gen Virol. 2000 Jun;81(Pt 6):1473-84. doi: 10.1099/0022-1317-81-6-1473.
2
Yellow head virus from Thailand and gill-associated virus from Australia are closely related but distinct prawn viruses.来自泰国的黄头病毒和来自澳大利亚的鳃相关病毒密切相关,但却是不同的对虾病毒。
Dis Aquat Organ. 1999 May 12;36(2):153-7. doi: 10.3354/dao036153.
3
A yellow head virus gene probe: nucleotide sequence and application for in situ hybridization.
实时逆转录环介导等温扩增快速检测对虾中的黄头病毒。
J Virol Methods. 2009 Dec;162(1-2):81-7. doi: 10.1016/j.jviromet.2009.07.018. Epub 2009 Jul 29.
4
Genomic organization, biology, and diagnosis of Taura syndrome virus and yellowhead virus of penaeid shrimp.对虾桃拉综合征病毒和黄头病毒的基因组结构、生物学特性及诊断
Adv Virus Res. 2004;63:353-421. doi: 10.1016/S0065-3527(04)63006-5.
5
Multiplex RT-nested PCR differentiation of gill-associated virus (Australia) from yellow head virus (Thailand) of Penaeus monodon.斑节对虾鳃相关病毒(澳大利亚)与黄头病毒(泰国)的多重逆转录巢式PCR鉴别
J Virol Methods. 2004 Apr;117(1):49-59. doi: 10.1016/j.jviromet.2003.11.018.
一种黄头病毒基因探针:核苷酸序列及其原位杂交应用
Dis Aquat Organ. 1999 Feb 26;35(3):165-73. doi: 10.3354/dao035165.
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A new RNA-friendly fixative for the preservation of penaeid shrimp samples for virological detection using cDNA genomic probes.一种新型的对RNA友好的固定剂,用于保存对虾样本,以便使用cDNA基因组探针进行病毒学检测。
J Virol Methods. 1997 Jul;66(2):227-36. doi: 10.1016/s0166-0934(97)00066-9.