Cowley J A, Dimmock C M, Wongteerasupaya C, Boonsaeng V, Panyim S, Walker P J
CRC for Aquaculture, CSIRO Tropical Agriculture, Indooroopilly, Australia.
Dis Aquat Organ. 1999 May 12;36(2):153-7. doi: 10.3354/dao036153.
Corresponding genomic regions of isolates of yellow head virus (YHV) from Thailand and gill-associated virus (GAV) from Australia were compared by RT-PCR and sequence analysis. PCR primers designed from sequences in the GAV ORF1b polyprotein gene amplified the corresponding 577 nucleotide region of the YHV genome. Comparison of the amplified region indicated 85.1% nucleotide and 95.8% amino acid sequence identity. YHV PCR primers designed to amplify a 135 nucleotide product previously described as a YHV diagnostic probe failed to amplify the corresponding product from GAV RNA. However, the cognate GAV sequence for this and another recently reported YHV sequence were located in an upstream region of the ORF1b gene. A comparison of these sequences indicated identities of 83.0 and 80.9% at the nucleotide level and 86.7 and 86.5% at the amino acid level, respectively. The data indicate that GAV and YHV are closely related but distinct viruses for which differential diagnostic probes can be applied.
通过逆转录聚合酶链反应（RT-PCR）和序列分析，对来自泰国的黄头病毒（YHV）分离株和来自澳大利亚的鳃相关病毒（GAV）的相应基因组区域进行了比较。根据GAV ORF1b多聚蛋白基因序列设计的PCR引物扩增出了YHV基因组相应的577个核苷酸区域。对扩增区域的比较表明，核苷酸序列同一性为85.1%，氨基酸序列同一性为95.8%。旨在扩增先前被描述为YHV诊断探针的135个核苷酸产物的YHV PCR引物未能从GAV RNA中扩增出相应产物。然而，该序列以及另一个最近报道的YHV序列的同源GAV序列位于ORF1b基因的上游区域。对这些序列的比较表明，核苷酸水平的同一性分别为83.0%和80.9%，氨基酸水平的同一性分别为86.7%和86.5%。数据表明，GAV和YHV是密切相关但不同的病毒，可应用差异诊断探针进行区分。
