Horakova Jana, Klicova Marketa, Erben Jakub, Klapstova Andrea, Novotny Vit, Behalek Lubos, Chvojka Jiri
Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 1402/2, 460 01 Liberec, Czech Republic.
Department of Nanomaterials in Natural Sciences, Institute of Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentska 1402/2, 460 01 Liberec, Czech Republic.
ACS Omega. 2020 Apr 7;5(15):8885-8892. doi: 10.1021/acsomega.0c00503. eCollection 2020 Apr 21.
Electrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.
由可生物降解的聚己内酯制成的电纺材料因其与细胞外基质的形态相似性而被广泛应用于各种组织工程和再生医学领域。然而，在临床实践中使用此类材料的主要前提是选择合适的灭菌技术。本研究致力于研究传统灭菌和消毒方法对通过无针电纺技术构建的纳米纤维聚己内酯层的影响。结果表明，过氧化氢等离子体处理导致纤维形态丧失并形成箔状物。然而，发现某些灭菌（环氧乙烷、伽马射线辐照和过氧乙酸）和消毒技术（乙醇和紫外线辐照）不会导致形态变化；因此，该研究调查了它们对热性能、分子量以及与成纤维细胞系相互作用的影响。结果表明，引导细胞黏附和增殖的表面性质比本体性质受到的影响更大。在纳米纤维支架上接种的成纤维细胞中，伽马射线辐照的聚己内酯的增殖率最高，而环氧乙烷灭菌后的增殖率最低。
