Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 1402/2, 460 01 Liberec, Czech Republic.
Biomed Phys Eng Express. 2020 Feb 24;6(2):025012. doi: 10.1088/2057-1976/ab756f.
Polycaprolactone (PCL) was electrospun with the addition of arginine (Arg), an α-amino acid that accelerates the healing process. The efficient needleless electrospinning technique was used for the fabrication of the nanofibrous layers. The materials produced consisted mainly of fibers with diameters of between 200 and 400 nm. Moreover, both microfibers and beads were present within the layers. Higher bead sizes were observed with the increased addition of arginine. The arginine content within the layers as well as the weight of the resultant electrospun materials were enhanced with the increased addition of arginine to the electrospinning solution (1, 5 and 10 wt%). The PCL + 1% Arg nanofibrous layer contained 5.67 ± 0.04% of arginine, the PCL + 5% Arg layer 22.66 ± 0.24% of arginine and the PCL + 10% Arg layer 37.33 ± 0.39% of arginine according to the results of the elemental analysis. A high burst release within 5 h of soaking was recorded for the PCL + 5% and PCL + 10% nanofibrous layers. However, the release rate of arginine from the PCL + 1% Arg was significantly slower, reaching a maximum level after 72 h of soaking. The resulting materials were hydrophobic. Hemocompatibility testing under static conditions revealed no effect on hemolysis following the addition of arginine and the prolongation of the prothrombin time with the increased addition of arginine, thus exerting an influence on the extrinsic and common pathway of coagulation activation. The results of the dynamic hemocompatibility assessment revealed that the numbers of blood cells and platelets were not affected significantly by the various electrospun samples during incubation. The TAT, β-thromboglobulin and SC5-b9 concentrations were characterized by a moderate increase in the PCL group compared to those of the control group. The presence of arginine resulted in a decrease in the investigated hemocompatibility markers. The PMN elastase levels were comparable with respect to all the groups.
聚己内酯（PCL）与精氨酸（Arg）共混进行静电纺丝，Arg 是一种加速愈合过程的α-氨基酸。高效的无针静电纺丝技术用于制备纳米纤维层。所制备的材料主要由直径在 200nm 到 400nm 之间的纤维组成。此外，层内还存在微纤维和珠粒。随着 Arg 添加量的增加，观察到珠粒尺寸更大。随着 Arg 向纺丝溶液中的添加量增加（1%、5%和 10%wt%），层内的 Arg 含量以及所得静电纺丝材料的重量都得到了提高。PCL+1%Arg 纳米纤维层含有 5.67±0.04%的 Arg，PCL+5%Arg 层含有 22.66±0.24%的 Arg，PCL+10%Arg 层含有 37.33±0.39%的 Arg，这是根据元素分析的结果。PCL+5%和 PCL+10%纳米纤维层在浸泡 5 小时内记录到高突释。然而，PCL+1%Arg 的 Arg 释放速度明显较慢，在浸泡 72 小时后达到最大值。所得材料是疏水性的。静态条件下的血液相容性测试表明，加入 Arg 后对溶血没有影响，随着 Arg 添加量的增加，凝血酶原时间延长，从而对凝血激活的外源性和共同途径产生影响。动态血液相容性评估的结果表明，在孵育过程中，各种静电纺丝样品对血细胞和血小板的数量没有显著影响。与对照组相比，PCL 组的 TAT、β-血栓球蛋白和 SC5-b9 浓度略有增加。存在 Arg 导致研究的血液相容性标志物减少。PMN 弹性蛋白酶水平与所有组相比都相当。
