Wongratanacheewin S, Sirisinha S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1987 Dec;18(4):511-20.
Antigens of Opisthorchis viverrini were identified and characterized by enzyme-linked immunosorbent assay, polyacrylamide gel electrophoresis following radioimmuno-precipitation, and indirect immunofluorescence. The immunoreactive protein with a relative molecular weight (Mr) of 89 kD was the predominating component of the parasite metabolic products. An immunofluorescence study showed it to be associated with parasite eggs, linings of the reproductive system and secretions therein. Protein of the surface tegument had Mr of greater than 116, 108, 64, 38, 34, 20 and 16-17 kD. The 16-17 kD surface molecule was the predominant protein, representing approximately 50% of the total protein in crude aqueous extracts of adult worms. However, it was poorly immunogenic when compared with the 89 kD molecule. Together with data presented previously, it appears that in addition to the 89 kD protein, several tegumental molecules are also specific for O. viverrini and have immuno-diagnostic potential.
通过酶联免疫吸附测定、放射免疫沉淀后的聚丙烯酰胺凝胶电泳以及间接免疫荧光法，对华支睾吸虫的抗原进行了鉴定和表征。相对分子质量（Mr）为89 kD的免疫反应性蛋白是该寄生虫代谢产物的主要成分。免疫荧光研究表明，它与寄生虫卵、生殖系统内衬及其分泌物有关。体表被膜蛋白的Mr大于116、108、64、38、34、20和16 - 17 kD。16 - 17 kD的表面分子是主要蛋白质，占成虫粗水提取物中总蛋白的约50%。然而，与89 kD分子相比，其免疫原性较差。结合之前呈现的数据，似乎除了89 kD蛋白外，几种被膜分子对华支睾吸虫也具有特异性，并且具有免疫诊断潜力。
