Wongratanacheewin S, Chawengkirttikul R, Bunnag D, Sirisinha S
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Parasitology. 1988 Feb;96 ( Pt 1):119-28. doi: 10.1017/s0031182000081701.
Antigenicity of adult Opisthorchis viverrini, metacercariae and eggs was investigated by radio-immunoprecipitation and polyacrylamide gel electrophoresis using sera from patients with opisthorchiasis and from hamsters experimentally infected with O. viverrini metacercariae. 125I-labelled aqueous somatic extract and in vitro metabolic products of adult worms, and the extracts of metacercariae and eggs were immunoprecipitated, electrophoresed and autoradiographed and major antigens were characterized. Although components with Mr of greater than 116, 89, 78 and 20 kD appeared to be specifically associated with the somatic extract of adult fluke, the 89 kD protein was the most prominent component found in the in vitro culture fluid of adult worms and the metacercarial extract. Hamsters, experimentally infected with O. viverrini, also showed the presence of antibody reactive with the 89 kD component within 15 days of infection. The potential of this antigen in immunodiagnosis is discussed.
利用华支睾吸虫病患者以及经华支睾吸虫囊蚴实验感染的仓鼠血清，通过放射免疫沉淀和聚丙烯酰胺凝胶电泳，研究了成年华支睾吸虫、囊蚴和虫卵的抗原性。对125I标记的成虫水溶性虫体提取物和体外代谢产物，以及囊蚴和虫卵提取物进行免疫沉淀、电泳和放射自显影，并对主要抗原进行了表征。虽然分子量大于116、89、78和20 kD的成分似乎与成虫虫体提取物有特异性关联，但89 kD蛋白是在成虫体外培养液和囊蚴提取物中发现的最突出成分。经华支睾吸虫实验感染的仓鼠在感染后15天内也显示出存在与89 kD成分反应的抗体。讨论了该抗原在免疫诊断中的潜力。
