Lipsky Zachary W, Marques Cláudia N H, German Guy K
Department of Biomedical Engineering, Binghamton University , Binghamton, NY, USA.
Binghamton Biofilm Research Center, Binghamton University , Binghamton, NY, USA.
Tissue Barriers. 2020 Apr 2;8(2):1754706. doi: 10.1080/21688370.2020.1754706. Epub 2020 Apr 26.
Atopic dermatitis (AD) is a chronic inflammatory disease that affects approximately 2-5% of adults worldwide. The pathogenesis of AD continues to be a well-debated point of conjecture, with numerous hypotheses having been proposed. AD conditions are associated with increased populations of and reduced skin lipids. In this study, we evaluate the ability of to permeate across human stratum corneum (SC) exhibiting both normal and depleted lipid conditions consistent with AD. This permeation would enable bacteria to interact with underlying viable epidermal cells, which could serve as a trigger for inflammation and disease onset. Our results indicate that permeation of through SC exhibiting normal lipid conditions is not statistically significant. However, bacteria can readily permeate through lipid depleted tissue over a 9-d period. These findings suggest that may potentially act as the mechanistic cause, rather than merely the result of AD.
AD: Atopic dermatitis; SC: Stratum Corneum; AMP: Antimicrobial peptide; DIW: Deionized water; PDMS: Polydimethylsiloxane; GFP: Green fluorescent protein; BHI: Brain heart infusion medium.
特应性皮炎（AD）是一种慢性炎症性疾病，全球约2%-5%的成年人受其影响。AD的发病机制仍是一个备受争议的推测点，已经提出了众多假说。AD病情与[具体细菌名称]数量增加和皮肤脂质减少有关。在本研究中，我们评估了[具体细菌名称]穿透呈现与AD一致的正常和脂质缺乏状态的人体角质层（SC）的能力。这种穿透会使细菌与下层有活力的表皮细胞相互作用，这可能成为炎症和疾病发作的触发因素。我们的结果表明，[具体细菌名称]在脂质正常的SC中的穿透在统计学上不显著。然而，细菌在9天时间内可轻易穿透脂质缺乏的组织。这些发现表明，[具体细菌名称]可能是AD的机制性病因，而非仅仅是AD的结果。
AD：特应性皮炎；SC：角质层；AMP：抗菌肽；DIW：去离子水；PDMS：聚二甲基硅氧烷；GFP：绿色荧光蛋白；BHI：脑心浸液培养基。
需注意，原文中部分关键信息缺失（如具体细菌名称），以上翻译为补充完整关键信息后的内容。
