College of Animal Science, Jilin University, No. 5333, Xi'an Road, Lvyuan District, Changchun, Jilin 130062, China; Changchun Qijian Biological Products Co., Ltd., No.1, Torch Road, High Tech Development Zone, Changchun, Jilin Province 130012, China.
College of Animal Science, Jilin University, No. 5333, Xi'an Road, Lvyuan District, Changchun, Jilin 130062, China.
Gene. 2020 Jul 30;749:144703. doi: 10.1016/j.gene.2020.144703. Epub 2020 Apr 24.
The repair of segmental bone defects and bone fractures is a clinical challenge involving high risk and postsurgical morbidity. Bone injury and partial bone tumor resection via traditional bone grafting result in high complications. Growth factors have been proposed as alternatives to promote bone repair and formation and circumvent these limitations. In this study, we classified different lengths of mechano growth factor (MGF) E peptides in different species and analyzed their effects on MC3T3-E1 cell proliferation, cell cycle, alkaline phosphatase (ALP) activity, differentiation-related factor expression, and cell mineralization. A rabbit bone injury model was constructed, and the repair function of MGF E peptide was verified by injecting the candidate MGF E peptide. We analyzed 52 different MGF-E peptides and classified them into the following four categories: T-MGF-25E, M-MGF-25E, T-MGF-19E, and M-MGF-19E. These peptides were synthesized for further study. T-MGF-19E peptide obviously promoted cell proliferation by regulating cell cycle after MGF E peptide treatment at 72 h. T-MGF-25E and T-MGF-19E peptide significantly promoted the differentiation of osteoblasts on day 14, and M-MGF-25E peptide promoted cell differentiation on day 7. T-MGF-19E, T-MGF-25E, and M-MGF-19E significantly promoted osteoblast mineralization, with T-MGF19E showing the most significant effect. These results implied that T-MGF19E peptide could remarkably promote MC3T3-E1 cell proliferation, differentiation, and mineralization. The rabbit bone defect model showed that the low-dose T-MGF-19E peptide significantly promoted bone injury healing, suggesting its promoting effect on the healing of bone injury.
节段性骨缺损和骨折的修复是一个临床挑战，涉及高风险和术后发病率。传统的骨移植导致骨损伤和部分骨肿瘤切除，并发症较高。生长因子已被提议作为促进骨修复和形成的替代品，以规避这些限制。在这项研究中，我们对不同物种的机械生长因子（MGF）E 肽进行了分类，并分析了它们对 MC3T3-E1 细胞增殖、细胞周期、碱性磷酸酶（ALP）活性、分化相关因子表达和细胞矿化的影响。构建了兔骨损伤模型，并通过注射候选 MGF E 肽验证了 MGF E 肽的修复功能。我们分析了 52 种不同的 MGF-E 肽，并将其分为以下四类：T-MGF-25E、M-MGF-25E、T-MGF-19E 和 M-MGF-19E。这些肽被合成用于进一步研究。在 MGF E 肽处理 72 小时后，T-MGF-19E 肽通过调节细胞周期明显促进细胞增殖。T-MGF-25E 和 T-MGF-19E 肽在第 14 天明显促进成骨细胞分化，M-MGF-25E 肽在第 7 天促进细胞分化。T-MGF-19E、T-MGF-25E 和 M-MGF-19E 显著促进成骨细胞矿化，其中 T-MGF19E 作用最显著。这些结果表明，T-MGF19E 肽可显著促进 MC3T3-E1 细胞增殖、分化和矿化。兔骨缺损模型表明，低剂量 T-MGF-19E 肽可显著促进骨损伤愈合，提示其对骨损伤愈合有促进作用。
