Department of Drug Sciences, University of Pavia, Viale Taramelli 12, 27100 Pavia, Italy.
FlaNat Research Italia Srl, Via Giuseppe di Vittorio 1, 20017 Rho (Milano), Italy.
Molecules. 2020 Apr 23;25(8):1958. doi: 10.3390/molecules25081958.
The reuse of byproducts from agricultural and food industries represents the key factor in a circular economy, whose interest has grown in the last two decades. Thus, the extraction of bioactives from agro-industrial byproducts is a potential source of valuable molecules. The aim of this work was to investigate the in vitro capacity of byproducts from a new Italian corn variety, named Moradyn, to inhibit the accumulation of advanced glycation end products (AGEs) involved in several chronic age-related disorders. In addition, the hypoglycemic effect of Moradyn was tested by in vitro enzymatic systems. A Moradyn phytocomplex and its purified anthocyanin fraction were able to inhibit fructosamine formation and exhibited antiglycative properties when tested using BSA-sugars and BSA-methylglyoxal assays. These properties could be attributed to the polyphenols, mainly anthocyanins and flavonols, detected by RP-HPLC-DAD-ESI-MS. Finally, a Moradyn phytocomplex was submitted to a simulated in vitro digestion process to study its bioaccessibility. Moradyn could be considered as a promising food ingredient in the context of typical type 2 diabetes risk factors and the study will continue in the optimization of the ideal formulation to preserve its bioactivities from digestion.
农业和食品工业副产物的再利用是循环经济的关键因素，在过去二十年中，其受到了越来越多的关注。因此，从农业副产物中提取生物活性物质是有价值分子的潜在来源。本研究的目的是从一种名为 Moradyn 的新型意大利玉米品种的副产物中，研究其体外抑制与多种慢性年龄相关疾病相关的晚期糖基化终产物（AGEs）积累的能力。此外，还通过体外酶系统测试了 Moradyn 的降血糖作用。Moradyn 植物复合物及其纯化的花色苷部分能够抑制果糖胺的形成，并在使用 BSA-糖和 BSA-甲基乙二醛测定法进行测试时表现出抗糖化特性。这些特性可归因于通过 RP-HPLC-DAD-ESI-MS 检测到的多酚，主要是花色苷和类黄酮。最后，将 Moradyn 植物复合物进行模拟体外消化过程，以研究其生物可利用性。在典型的 2 型糖尿病风险因素背景下，Moradyn 可以被认为是一种很有前途的食品成分，并且将继续优化理想配方以保护其消化过程中的生物活性。
