Moretto Giulia, Colombo Raffaella, Negri Stefano, Cena Hellas, Vailati Lorena, Papetti Adele
Drug Sciences Department, University of Pavia, 27100 Pavia, Italy.
National Biodiversity Future Center (NBFC), 90133 Palermo, Italy.
Nutrients. 2025 Mar 7;17(6):935. doi: 10.3390/nu17060935.
: Italy's plant biodiversity, characterized by many plant species, is an important source of bioactive secondary metabolites that help reduce the risk of the development of advanced glycation end product (AGE)-related diseases. AGEs are involved in various diseases, such as diabetes, cardiovascular, and neurodegenerative disorders. Therefore, the aim of the study was to investigate the antiglycative, hypoglycemic, and neuroprotective properties of nine edible plant extracts using different in vitro assays. : The ability of the extracts to counteract AGE formation was evaluated at different stages of the glycation reaction using in vitro systems based on the determination of Amadori products and the co-incubation of a model protein with a dicarbonyl compound under different experimental conditions. In addition, the extracts' methylglyoxal (MGO) and glyoxal (GO) trapping ability was investigated. Hypoglycemic activity was assessed by measuring α-amylase inhibition, while the neuroprotective effects were explored by testing amyloid β peptide 1-42 (Aβ1-42) fibrillogenesis inhibition. : All extracts generally had a dose-related capacity for the inhibition of AGE formation, mainly at the intermediate stage of the glycation reaction; high trapping capacity against MGO and GO; and promising hypoglycemic properties. In addition, they affected the fibrillogenesis process by reducing mature amyloid fibril formation and altering fibril morphology. : All tested extracts had promising anti-fibrillogenic properties. extract was the most active among the tested plant species given its antiglycative activity (about 80% inhibition of AGE formation), trapping capacity against MGO and GO (almost 100%), hypoglycemic effects (66.20 ± 0.88%), and anti-fibrillogenic effects (69.00 ± 4.49% inhibition), indicating its suitability in the management of AGE-related diseases and for the potential development of a novel food ingredient.
意大利的植物生物多样性以众多植物物种为特征，是生物活性次生代谢产物的重要来源，有助于降低晚期糖基化终产物（AGE）相关疾病的发病风险。AGEs与多种疾病有关，如糖尿病、心血管疾病和神经退行性疾病。因此，本研究的目的是使用不同的体外试验来研究九种可食用植物提取物的抗糖基化、降血糖和神经保护特性。：使用基于Amadori产物测定的体外系统以及在不同实验条件下将模型蛋白与二羰基化合物共同孵育，在糖基化反应的不同阶段评估提取物对抗AGE形成的能力。此外，还研究了提取物捕获甲基乙二醛（MGO）和乙二醛（GO）的能力。通过测量α-淀粉酶抑制来评估降血糖活性，而通过测试淀粉样β肽1-42（Aβ1-42）纤维形成抑制来探索神经保护作用。：所有提取物通常都具有与剂量相关的抑制AGE形成的能力，主要在糖基化反应的中间阶段；对MGO和GO具有高捕获能力；以及有前景的降血糖特性。此外，它们通过减少成熟淀粉样纤维的形成和改变纤维形态来影响纤维形成过程。：所有测试的提取物都具有有前景的抗纤维形成特性。在所测试的植物物种中，[提取物名称]是最具活性的，因其具有抗糖基化活性（约80%抑制AGE形成）、对MGO和GO的捕获能力（几乎100%）、降血糖作用（66.20±0.88%）和抗纤维形成作用（69.00±4.49%抑制），表明其适用于管理AGE相关疾病以及潜在开发新型食品成分。
