Lu Zengkui, Liu Jianbin, Han Jilong, Yang Bohui
Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
Sheep Breeding Engineering Technology Research Center of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China.
Animals (Basel). 2020 Apr 23;10(4):739. doi: 10.3390/ani10040739.
Bone morphogenetic protein 2 () is strongly selected in both fat-tailed and thin-tailed sheep and may be a candidate gene for sheep tail type selection. However, the mechanism of action of in sheep tail fat deposition remains unclear. This study investigated genetic variation and haplotype combinations of the gene in sheep with different tail types, aiming to reveal the molecular mechanism of in sheep tail fat deposition. We detected a total of three single nucleotide polymorphisms (SNPs) (g.48401619 T > A, g.48401272 C > A, and g.48401136 C > T) among 533 sheep. The alleles and genotype frequencies of these SNPs were in Hardy-Weinberg equilibrium and showed significant correlations with tail length. Linkage disequilibrium existed between the g.48401272 C > A and g.48401136 C > T sites, where CACT was the predominant genotype. At the cellular level, the expression levels of peroxisome proliferator-activated receptor gamma () and lipoprotein lipase () were upregulated after overexpression; there were significantly higher levels of than controls at 0 d and 1 d, and of than controls at 1 d and 7 d. These results indicate that the gene may participate in sheep tail fat deposition and could be used for molecular-marker-assisted selection of sheep tail type.
骨形态发生蛋白2（BMP2）在肥尾羊和瘦尾羊中均受到强烈选择，可能是绵羊尾型选择的候选基因。然而，BMP2在绵羊尾部脂肪沉积中的作用机制尚不清楚。本研究调查了不同尾型绵羊中BMP2基因的遗传变异和单倍型组合，旨在揭示BMP2在绵羊尾部脂肪沉积中的分子机制。我们在533只绵羊中共检测到三个单核苷酸多态性（SNP）（g.48401619 T>A、g.48401272 C>A和g.48401136 C>T）。这些SNP的等位基因和基因型频率处于哈迪-温伯格平衡，且与尾长呈显著相关。g.48401272 C>A和g.48401136 C>T位点之间存在连锁不平衡，其中CACT是主要基因型。在细胞水平上，过表达BMP2后，过氧化物酶体增殖物激活受体γ（PPARγ）和脂蛋白脂肪酶（LPL）的表达水平上调；在第0天和第1天，BMP2的水平显著高于对照组，在第1天和第7天，PPARγ的水平显著高于对照组。这些结果表明，BMP2基因可能参与绵羊尾部脂肪沉积，可用于绵羊尾型的分子标记辅助选择。
