College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China.
Key Laboratory of Farm Animal Genetic Resources Exploration and Breeding of Shanxi Province, Jinzhong 030801, China.
Int J Mol Sci. 2023 Oct 4;24(19):14892. doi: 10.3390/ijms241914892.
Fat deposition involves the continuous differentiation of adipocytes and lipid accumulation. Studies have shown that microRNA miR-136 and 17β-hydroxysteroid dehydrogenase type 12 () play important roles in lipid accumulation. However, the regulatory mechanism through which miR-136 targets during ovine adipogenesis remains unclear. This study aimed to elucidate the role of miR-136 and in adipogenesis and their relationship in ovine adipose-derived stromal vascular fractions (SVFs). The target relationship between miR-136 and was predicted and confirmed using bioinformatics and a dual-luciferase reporter assay. The results showed that miR-136 promoted proliferation and inhibited adipogenic differentiation of ovine SVFs. We also found that inhibited proliferation and promoted adipogenic differentiation of ovine SVFs. Collectively, our results indicate that miR-136 facilitates proliferation and attenuates adipogenic differentiation of ovine SVFs by targeting . These findings provide a theoretical foundation for further elucidation of the regulatory mechanisms of lipid deposition in sheep.
脂肪沉积涉及脂肪细胞的不断分化和脂质积累。研究表明，微小 RNA miR-136 和 17β-羟甾类脱氢酶 12（）在脂质积累中发挥重要作用。然而，miR-136 靶向在绵羊脂肪生成中的调控机制尚不清楚。本研究旨在阐明 miR-136 和 在脂肪生成中的作用及其在绵羊脂肪组织来源基质血管部分（SVFs）中的关系。使用生物信息学和双荧光素酶报告基因检测预测和证实了 miR-136 和 之间的靶关系。结果表明，miR-136 促进绵羊 SVFs 的增殖并抑制其脂肪生成分化。我们还发现 抑制绵羊 SVFs 的增殖并促进其脂肪生成分化。综上所述，我们的结果表明，miR-136 通过靶向 促进绵羊 SVFs 的增殖并减弱其脂肪生成分化。这些发现为进一步阐明绵羊脂质沉积的调控机制提供了理论基础。
