Regional mapping of the creatine kinase b (CKBB) gene in rabbit (Oryctolagus cuniculus) and man using a rat cDNA probe.

Using a rat creatine kinase (brain form) cDNA clone for in situ hybridization, we have localized the gene in both the human and the rabbit complement. An analysis of the data shows that the locus in the human is at 14q32, confirming previous assignments based on somatic hybridization studies and Southern blot analysis. In the rabbit, significant accumulation on 20q13----qter with the predominant labeling at the end of the chromosome provides evidence for the localization of the gene at this site. The heterologous hybridizations of a rat probe to both human and rabbit metaphases underscore the highly conserved nature of the sequences for this enzyme.