Department of Medicine, Duke Molecular Physiology Institute, Duke University School of Medicine, Durham, NC, USA.
Department of Medicine, Division of Rheumatology, Duke University School of Medicine, Durham, NC, USA.
Cartilage. 2021 Dec;13(1_suppl):1741S-1748S. doi: 10.1177/1947603520916523. Epub 2020 Apr 27.
To evaluate effects of physical activity and food consumption on plasma concentrations of free and total transforming growth factor beta-1 (TGF-β1), beta-2 (TGF-β2), and beta-3 (TGF-β3) in individuals with knee osteoarthritis (OA).
Participants ( = 40 in 2 cohorts of 20; mean age 70 years) with radiographic knee OA were admitted overnight for serial blood sampling. Cohorts 1 and 2 assessed the impacts of food intake and activity, respectively, on TGF-β concentrations. Cohort 1 blood draws included 2 hours postprandial the evening of day 1 (T3), fasting before rising on day 2 (T0), nonfasting 1 hour after rising (T1B), and 4 hours after rising (T2). Cohort 2 blood draws included T3, T0, fasting 1 hour after rising and performing activities of daily living (T1A), and nonfasting 2 hours after rising (T1B). By sandwich ELISAs, we quantified plasma free and total TGF-β1 concentrations in all samples, and plasma total TGF-β2 and TGF-β3 in cohort 2.
Free TGF-β1 represented a small fraction of the total systemic concentration (mean 0.026%). In cohort 2, free and total TGF-β1 and total TGF-β2 concentration significantly increased in fasting samples collected after an hour (T1A) of activities of daily living (free TGF-β1: = 0.006; total TGF-β1: < 0.001; total TGF-β2: = 0.001). Total TGF-β3 increased nonsignificantly following activity ( = 0.590) and decreased ( = 0.035) after food consumption while resting (T1B).
Increased plasma concentrations of TGF-β with physical activity suggests activity should be standardized prior to TGF-β1 analyses.
评估体力活动和食物摄入对膝骨关节炎（OA）患者血浆游离和总转化生长因子β-1（TGF-β1）、β-2（TGF-β2）和β-3（TGF-β3）浓度的影响。
纳入了 40 名影像学 OA 患者（2 个队列各 20 名，平均年龄 70 岁），患者入院过夜进行连续采血。队列 1 和 2 分别评估了饮食摄入和活动对 TGF-β 浓度的影响。队列 1 的采血包括第 1 天（T3）的晚餐后 2 小时、第 2 天清晨空腹（T0）、醒来后 1 小时非空腹（T1B）和醒来后 4 小时（T2）。队列 2 的采血包括 T3、T0、醒来后 1 小时空腹并进行日常活动（T1A）和醒来后 2 小时非空腹（T1B）。采用夹心 ELISA 法检测所有样本中的游离和总 TGF-β1 浓度，以及队列 2 中的总 TGF-β2 和 TGF-β3 浓度。
游离 TGF-β1 仅占总系统浓度的一小部分（平均 0.026%）。在队列 2 中，空腹样本在日常活动 1 小时（T1A）后采集时，游离和总 TGF-β1 以及总 TGF-β2 浓度显著升高（游离 TGF-β1： = 0.006；总 TGF-β1：<0.001；总 TGF-β2： = 0.001）。总 TGF-β3 在活动后无显著升高（ = 0.590），而在休息时（T1B）进食后降低（ = 0.035）。
与体力活动相关的 TGF-β 血浆浓度升高提示在进行 TGF-β1 分析前应标准化活动。
