Morgridge Institute for Research, Madison, Wisconsin, USA; Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, Madison, Wisconsin, USA; Department of Medical Physics, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Ultrasound Med Biol. 2020 Jul;46(7):1808-1814. doi: 10.1016/j.ultrasmedbio.2020.03.010. Epub 2020 Apr 25.
Efforts to develop quantitative ultrasound biomarkers would benefit from comparisons between ultrasound data and higher-resolution images of the tissue microstructure, such as from optical microscopy. However, only a few studies have used these methods for multiscale imaging because it is difficult to register low-resolution (>100 μm) ultrasound images to high-resolution microscopy images. To address this need, we have designed a 3-D-printed registration phantom that is made of a hard fluorescent resin, fits into a glass-bottom dish and can be used to calculate a coordinate system transform between ultrasound and optical microscopy. We report the phantom design, a registration protocol and an example registration using 18.5-MHz ultrasound and second harmonic generation microscopy. We evaluate the registration precision, achieving standard deviations smaller than the ultrasound resolution across all axes, and illustrate on a mouse mammary gland that this method yields results superior to those of manual landmark registration.
努力开发定量超声生物标志物将受益于超声数据与组织微观结构的更高分辨率图像（如光学显微镜图像）之间的比较。然而，由于很难将低分辨率（> 100 μm）超声图像与高分辨率显微镜图像配准，因此只有少数研究使用这些方法进行多尺度成像。为了解决这一需求，我们设计了一种 3D 打印的配准体模，它由硬荧光树脂制成，可放入玻璃底培养皿中，并可用于计算超声和光学显微镜之间的坐标系变换。我们报告了体模设计、配准协议和使用 18.5MHz 超声和二次谐波产生显微镜的示例配准。我们评估了配准精度，在所有轴上均达到了小于超声分辨率的标准偏差，并在小鼠乳腺上说明了该方法的结果优于手动地标配准。
