Department of Physics, University of Cambridge, Cambridge, UK.
Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK.
Nat Biomed Eng. 2022 May;6(5):541-558. doi: 10.1038/s41551-022-00890-6. Epub 2022 May 27.
A lack of accepted standards and standardized phantoms suitable for the technical validation of biophotonic instrumentation hinders the reliability and reproducibility of its experimental outputs. In this Perspective, we discuss general criteria for the design of tissue-mimicking biophotonic phantoms, and use these criteria and state-of-the-art developments to critically review the literature on phantom materials and on the fabrication of phantoms. By focusing on representative examples of standardization in diffuse optical imaging and spectroscopy, fluorescence-guided surgery and photoacoustic imaging, we identify unmet needs in the development of phantoms and a set of criteria (leveraging characterization, collaboration, communication and commitment) for the standardization of biophotonic instrumentation.
缺乏可接受的标准和适用于生物光子仪器技术验证的标准化体模，阻碍了其实验结果的可靠性和可重复性。在本观点中，我们讨论了设计组织模拟生物光子体模的一般标准，并使用这些标准和最新进展，批判性地回顾了关于体模材料和体模制作的文献。通过聚焦于漫射光学成像和光谱学、荧光引导手术和光声成像的标准化的代表性实例，我们确定了在体模开发和生物光子仪器标准化方面的一些未满足的需求，以及一套标准（利用特性描述、协作、交流和承诺）。
