Sunway University, School of Healthcare and Medical Sciences, Department of Medical Sciences, Jalan Universiti, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia.
Malays J Pathol. 2020 Apr;42(1):85-90.
Autophagy is a mechanism that degrades large damaged organelles and misfolded proteins to maintain the homeostasis in all cells. It plays double-faceted roles in tumourigenesis and prevention of various cancers. In our side observation of investigating the prognostic value of autophagy in colorectal cancer (CRC), we found high expression of autophagy proteins (LC3A, LC3B, and p62/SQSTM1) in the colonic ganglion cells. To our best understanding, this is the first paper reporting such finding.
Formalin-fixed paraffin-embedded (FFPE) CRC tissues blocks were retrieved and confirmed by haematoxylin & eosin (H&E) staining. Immunohistochemistry (IHC) targeting autophagy proteins (LC3A, LC3B, and p62/SQSTM1) was then performed followed by pathological examination.
All three autophagy proteins were present in both normal and tumour tissues of CRC patients. Interestingly, high expression of autophagy proteins in colonic ganglion cells was consistently seen regardless of tissue type (normal or cancer) or tumour site (caecum, ascending, transverse, descending, sigmoid colon and rectum).
This work highlights the high autophagic activities in human colonic ganglion cells.
自噬是一种降解大型受损细胞器和错误折叠蛋白质的机制，以维持所有细胞的内稳态。它在肿瘤发生和预防各种癌症中发挥着双重作用。在我们调查自噬在结直肠癌（CRC）中的预后价值的研究中，我们发现自噬蛋白（LC3A、LC3B 和 p62/SQSTM1）在结肠神经节细胞中的高表达。据我们所知，这是第一篇报道这种发现的论文。
检索并通过苏木精和伊红（H&E）染色确认福尔马林固定石蜡包埋（FFPE）CRC 组织块。然后进行针对自噬蛋白（LC3A、LC3B 和 p62/SQSTM1）的免疫组织化学（IHC）检测，随后进行病理检查。
所有三种自噬蛋白均存在于 CRC 患者的正常和肿瘤组织中。有趣的是，无论组织类型（正常或癌症）或肿瘤部位（盲肠、升结肠、横结肠、降结肠、乙状结肠和直肠）如何，结肠神经节细胞中自噬蛋白的高表达均一致存在。
这项工作强调了人类结肠神经节细胞中高自噬活性的存在。
