Berezowska Sabina, Galván José A
Institute of Pathology, University of Bern, Murtenstrasse 31, 3010, Bern, Switzerland.
Methods Mol Biol. 2017;1560:189-194. doi: 10.1007/978-1-4939-6788-9_13.
Autophagy is a highly conserved cellular mechanism of "self digestion," ensuring cellular homeostasis, and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy.LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy, and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
自噬是一种高度保守的“自我消化”细胞机制，可确保细胞内稳态，并在包括癌症在内的多种疾病中发挥作用。作为一种应激反应机制，它也可能参与细胞对治疗的反应。微管相关蛋白1轻链3（LC3）和聚集体结合蛋白1（p62/SQSTM1）是监测自噬最常用的标志物，可通过免疫组织化学在福尔马林固定石蜡包埋组织中进行可视化检测。在此，我们描述了一种经过验证的染色方案，该方案使用许多常规病理实验室都有的自动染色系统，能够在标准化条件下进行高通量染色。
