免疫组化检测福尔马林固定石蜡包埋组织中的自噬标志物 LC3 和 p62/SQSTM1。

Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

机构信息

Department of Laboratory Medicine and Pathology, Institute of Pathology, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

Institute of Pathology, University of Bern, Bern, Switzerland.

出版信息

Methods Mol Biol. 2023;2566:133-139. doi: 10.1007/978-1-0716-2675-7_10.

DOI:10.1007/978-1-0716-2675-7_10

PMID:36152247

Abstract

Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.

摘要

自噬是一种高度保守的“自我消化”细胞机制，可确保细胞内环境稳定，并在包括癌症在内的许多疾病中发挥作用。作为一种应激反应机制，它也可能参与细胞对治疗的反应。LC3 和自噬体相关蛋白 1 (p62/SQSTM1) 是最常用于监测自噬的标志物之一，可通过免疫组织化学在福尔马林固定和石蜡包埋组织中观察到。本文描述了一种使用许多常规病理实验室都有的自动化染色系统进行验证的染色方案，可在标准化条件下实现高通量染色。