Antikinetoplastid Activity of Indolocarbazoles from .

Chagas disease and leishmaniasis are neglected tropical diseases caused by kinetoplastid parasites of and genera that affect poor and remote populations in developing countries. These parasites share similar complex life cycles and modes of infection. It has been demonstrated that the particular group of phosphorylating enzymes, protein kinases (PKs), are essential for the infective mechanisms and for parasite survival. The natural indolocarbazole staurosporine (STS, ) has been extensively used as a PKC inhibitor and its antiparasitic effects described. In this research, we analyze the antikinetoplastid activities of three indolocarbazole (ICZs) alkaloids of the family of staurosporine STS, -, and the commercial ICZs rebeccamycin (), K252a (), K252b (), K252c (), and arcyriaflavin A () in order to establish a plausive approach to the mode of action and to provide a preliminary qualitative structure-activity analysis. The most active compound was 7-oxostaurosporine (7OSTS, ) that showed IC values of 3.58 ± 1.10; 0.56 ± 0.06 and 1.58 ± 0.52 µM against and and a Selectivity Index (CC/IC) of 52 against amastigotes of compared to the J774A.1 cell line of mouse macrophages.