Borcherding Kai, Marx Dennis, Gätjen Linda, Specht Uwe, Salz Dirk, Thiel Karsten, Wildemann Britt, Grunwald Ingo
Department of Adhesive Bonding Technology and Surfaces, Fraunhofer Institute for Manufacturing Technology and Advanced Materials (IFAM), 28359 Bremen, Germany.
Julius Wolff Institute, BIH Center for Regenerative Therapies, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 13353 Berlin, Germany.
Materials (Basel). 2020 Apr 24;13(8):2000. doi: 10.3390/ma13082000.
Improved implant osteointegration offers meaningful potential for orthopedic, spinal, and dental implants. In this study, a laser treatment was used for the structuring of a titanium alloy (Ti6Al4V) surface combined with a titanium dioxide coating, whereby a porous surface was created. The objective was to characterize the pore structure shape, treatment-related metallographic changes, cytocompatibility, and attachment of osteoblast-like cells (MG-63). The treatment generated specific bottleneck pore shapes, offering the potential for the interlocking of osteoblasts within undercuts in the implant surface. The pore dimensions were a bottleneck diameter of 27 µm (SD: 4 µm), an inner pore width of 78 µm (SD: 6 µm), and a pore depth of 129 µm (SD: 8 µm). The introduced energy of the laser changed the metallic structure of the alloy within the heat-affected region (approximately 66 µm) without any indication of a micro cracking formation. The phase of the alloy (microcrystalline alpha + beta) was changed to a martensite alpha phase in the surface region and an alpha + beta phase in the transition region between the pores. The MG-63 cells adhered to the structured titanium surface within 30 min and grew with numerous filopodia over and into the pores over the following days. Cell viability was improved on the structured surface compared to pure titanium, indicating good cytocompatibility. In particular, the demonstrated affinity of MG-63 cells to grow into the pores offers the potential to provide significantly improved implant fixation in further studies.
改善种植体骨整合为骨科、脊柱和牙科植入物提供了有意义的潜力。在本研究中，采用激光处理对结合二氧化钛涂层的钛合金（Ti6Al4V）表面进行结构化处理，从而形成多孔表面。目的是表征孔结构形状、与处理相关的金相变化、细胞相容性以及成骨样细胞（MG-63）的附着情况。该处理产生了特定的瓶颈状孔形状，为成骨细胞在植入物表面底切内的联锁提供了潜力。孔尺寸为瓶颈直径27 µm（标准差：4 µm）、内孔宽度78 µm（标准差：6 µm）和孔深129 µm（标准差：8 µm）。引入的激光能量改变了热影响区域（约66 µm）内合金的金属结构，没有任何微裂纹形成的迹象。合金的相（微晶α+β）在表面区域转变为马氏体α相，在孔之间的过渡区域转变为α+β相。MG-63细胞在30分钟内粘附到结构化钛表面，并在随后几天内长出许多丝状伪足并延伸到孔中。与纯钛相比，结构化表面上的细胞活力得到改善，表明具有良好的细胞相容性。特别是，MG-63细胞向孔内生长所表现出的亲和力为进一步研究提供了显著改善植入物固定的潜力。
